التفاصيل البيبلوغرافية
| العنوان: |
Atropine can induce autophagy independent of the M3 muscarinic acetylcholine receptor. |
| المؤلفون: |
Hai-Chang Yin1,2, Wen-Zhu Zhuang2, Xin-Jie Jiang2 Jiangxinjie777@163.com, Di Liu1 liudi1963@163.com |
| المصدر: |
Traditional Medicine Research. Jul2024, Vol. 9 Issue 7, p1-7. 7p. |
| مصطلحات موضوعية: |
*MUSCARINIC acetylcholine receptors, *ATROPINE, *NICOTINIC acetylcholine receptors, *AUTOPHAGY, *MYOCARDIUM |
| مستخلص: |
Background: No other effects of atropine other than as an antagonist of muscarinic acetylcholine receptor (mAChR) have been found. Methods: In this study, human kidney epithelial cells were treated with different physiological regulators. Results: Subsequently, it was found that atropine could significantly induce autophagy as demonstrated by the appearance of autophagosome-like double- or single-membrane vesicles in the cytoplasm of host cells and the number of GFP-LC3 dots. In addition, increased conversion of the autophagy marker protein LC3-I and LC3-II and increased p62/SQSTM1 indicated incomplete autophagy. In addition, atropine induced autophagosome levels in a dose-dependent manner within a certain concentration range in human kidney epithelial cells. In atropine-treated mouse skeletal muscle cells containing nicotinic acetylcholine receptors and rat cardiac muscle cells containing mAchR, atropine induced autophagy in mouse skeletal muscle cells but not in rat cardiac muscle cells. Furthermore, atropine did not induce autophagy in tissue cells containing mAchR in vivo but did in tissue cells not containing mAchR. Conclusion: This study expands the application and understanding of atropine's action mechanism in the field of medicine. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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