التفاصيل البيبلوغرافية
| العنوان: |
Tomato (Lycopersicon esculentum cv. Pik-Red) Leaf Carboxypeptidase: Identification, N-terminal Sequence, Stress-Regulation, and Specific Localization in the Paraveinal Mesophyll Vacuoles |
| المؤلفون: |
Mehta, Roshni A., Warmbardt, Robert D., Mattoo, Autar K. |
| بيانات النشر: |
Oxford University Press |
| سنة النشر: |
1996 |
| المجموعة: |
HighWire Press (Stanford University) |
| مصطلحات موضوعية: |
research-article |
| الوصف: |
Wounding of tomato ( Lycopersicon esculentum L.) leaves causes systemic induction of a serine-type carboxypeptidase activity. We find this activity to be present in several isoforms. Antibodies raised against the leaf carboxypeptidase inhibited the enzyme activity and the immunoprecipitates were resolved into a 69-kDa polypeptide and a doublet of 35/37-kDa proteins on SDS-PAGE. Immunoblot analysis of the leaf proteins also immunodecorated the 69-kDa and 35/37-kDa proteins. Amino acid sequence analysis of the amino-terminus of the tomato leaf 69-kDa carboxypeptidase showed it to be similar to the barley A-chain carboxypeptidase I [Sorenson et al. (1986) Carlsberg Res. Commun . 51: 475], sharing Ala as the N-terminus and the sequences, AlaProGln and LeuProGlyPhe. Superimposition of a chemical stress (copper treatment) on wounding apparently lowered wound-induced carboxypeptidase activity in the leaf, suggesting that cupric ions might interact with the wound signal. Immunogold electron microscopy indicated that the leaf carboxypeptidase was specifically localized within the inclusions of vacuoles of vascular parenchyma cells. In cupric ion-treated tissues, carboxypeptidase was found redistributed to other parts of the cell, indicating that this treatment, but not wounding, causes general vacuolar membrane damage. |
| نوع الوثيقة: |
text |
| وصف الملف: |
text/html |
| اللغة: |
English |
| العلاقة: |
http://pcp.oxfordjournals.org/cgi/content/short/37/6/806Test |
| الإتاحة: |
http://pcp.oxfordjournals.org/cgi/content/short/37/6/806Test |
| حقوق: |
Copyright (C) 1996, The Japanese Society of Plant Physiologists |
| رقم الانضمام: |
edsbas.416A604A |
| قاعدة البيانات: |
BASE |
