التفاصيل البيبلوغرافية
| العنوان: |
Postsynthetic Modification of the Magnetic Zirconium–Organic Framework for Efficient and Rapid Solid-Phase Extraction of DNA |
| المؤلفون: |
Wang Pan (2231176), Xuemei Wang (39992), Xueping Ma (2841272), Ya’nan Chu (11570339), Shuyun Pang (11570342), Yuqiu Chen (6414422), Xiaoxiang Guan (420958), Bingjie Zou (1662283), Yanzi Wu (11372663), Guohua Zhou (724981) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, swab preservation solution, suitable size modulated, excellent structural tunability, adsorption mechanism indicated, highly specific extraction, higher extraction efficiency, 3 , 2 , based extraction method, dna recovery investigation, 4 , effective dna extraction, phase extraction, extraction process, 4 %), based mofs, viral dna |
| الوصف: |
In recent years, several approaches have been applied to modify metal–organic frameworks (MOFs) owing to their excellent structural tunability such as higher extraction efficiency than that of primitive crystals. Herein, Zr-based MOFs (UiO-66-NH 2 ) with a suitable size modulated by acetic acid were successfully synthesized for effective DNA extraction. The bonding conformations and adsorption mechanism indicated a high affinity between UiO-66-NH 2 and the DNA molecules. Furthermore, Fe 3 O 4 nanoparticles were immobilized on the UiO-66-NH 2 surface to allow MOFs with magnetism. The magnetic zirconium–organic framework (MZMOF) retained the intact structure of MOFs and simplified subsequent extraction operations. In the DNA recovery investigation, MZMOF showed high recovery efficiency for both short-stranded DNA (90.4%) and pseudovirus DNA (95.1%). In addition, it showed superior DNA extraction efficiency from plasma (57.6%) and swab preservation solution (86.5%). The prepared MZMOF was employed for highly specific extraction of viral DNA and cfDNA from samples. To further simplify the extraction process, MZMOF was applied to immiscible phase filtration assisted by a surface tension (IFAST) chip for facilitating rapid DNA extraction with sensitive point-of-care testing. The developed MZMOF-based extraction method has significant potential for increasing the demand for rapid and efficient nucleic acid extraction. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Postsynthetic_Modification_of_the_Magnetic_Zirconium_Organic_Framework_for_Efficient_and_Rapid_Solid-Phase_Extraction_of_DNA/16819124Test |
| DOI: |
10.1021/acsami.1c12622.s001 |
| الإتاحة: |
https://doi.org/10.1021/acsami.1c12622.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.57629F87 |
| قاعدة البيانات: |
BASE |
