دورية أكاديمية
The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair
| العنوان: | The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair |
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| المؤلفون: | Wallis S. S., Ventimiglia L. N., Otigbah E., Infante E., Cuesta-Geijo M. A., Kidiyoor G. R., Carbajal M. A., Fleck R. A., Foiani M., Garcia-Manyes S., Martin-Serrano J., Agromayor M. |
| المساهمون: | S.S. Walli, L.N. Ventimiglia, E. Otigbah, E. Infante, M.A. Cuesta-Geijo, G.R. Kidiyoor, M.A. Carbajal, R.A. Fleck, M. Foiani, S. Garcia-Manye, J. Martin-Serrano, M. Agromayor |
| بيانات النشر: | Cell Press |
| سنة النشر: | 2021 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | ESCRT, LINC complex, mechanosensing, membrane repair, nuclear envelope, Actin, Cell Movement, Cell Nucleu, Cytoskeleton, Endosomal Sorting Complexes Required for Transport, HeLa Cell, Human, Mechanical Phenomena, Microfilament Protein, Nerve Tissue Protein, Settore BIO/11 - Biologia Molecolare |
| الوصف: | Transient nuclear envelope ruptures during interphase (NERDI) occur due to cytoskeletal compressive forces at sites of weakened lamina, and delayed NERDI repair results in genomic instability. Nuclear envelope (NE) sealing is completed by endosomal sorting complex required for transport (ESCRT) machinery. A key unanswered question is how local compressive forces are counteracted to allow efficient membrane resealing. Here, we identify the ESCRT-associated protein BROX as a crucial factor required to accelerate repair of the NE. Critically, BROX binds Nesprin-2G, a component of the linker of nucleoskeleton and cytoskeleton complex (LINC). This interaction promotes Nesprin-2G ubiquitination and facilitates the relaxation of mechanical stress imposed by compressive actin fibers at the rupture site. Thus, BROX rebalances excessive cytoskeletal forces in cells experiencing NE instability to promote effective NERDI repair. Our results demonstrate that BROX coordinates mechanoregulation with membrane remodeling to ensure the maintenance of nuclear-cytoplasmic compartmentalization and genomic stability. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34818527; info:eu-repo/semantics/altIdentifier/wos/WOS:000729283100007; volume:56; issue:23; firstpage:3192; lastpage:3202.e8; numberofpages:11; journal:DEVELOPMENTAL CELL; https://hdl.handle.net/2434/915371Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85120169353 |
| DOI: | 10.1016/j.devcel.2021.10.022 |
| الإتاحة: | https://doi.org/10.1016/j.devcel.2021.10.022Test https://hdl.handle.net/2434/915371Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.203353E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.devcel.2021.10.022 |
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