دورية أكاديمية
Nasal allergen neutralising IgG4 antibodies block IgE-mediated responses: novel biomarker of subcutaneous grass pollen immunotherapy
| العنوان: | Nasal allergen neutralising IgG4 antibodies block IgE-mediated responses: novel biomarker of subcutaneous grass pollen immunotherapy |
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| المؤلفون: | Shamji, MH, Kappen, J, Abubakar-Waziri, H, Zhang, J, Steveling, E, Watchman, S, Kouser, L, Eifan, A, Switzer, A, Varrichi, G, Marone, G, Couto-Francisco, NC, Calderon, M, Durham, SR |
| المساهمون: | Medical Research Council (MRC) |
| المصدر: | 1076 ; 1067 |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2018 |
| المجموعة: | Imperial College London: Spiral |
| مصطلحات موضوعية: | Biomarkers of allergen immunotherapy, IgA(1), IgA(2), IgE-FAB, IgG(4), Subcutaneous immunotherapy, allergic rhinoconjunctivitis with and without asthma, blocking antibodies, Science & Technology, Life Sciences & Biomedicine, Allergy, Immunology, IgE-facilitated allergen binding, REGULATORY B-CELLS, QUALITY-OF-LIFE, CLINICAL-EFFICACY, BINDING, VACCINATION, INHIBITION, ASTHMA, RHINOCONJUNCTIVITIS, VALIDATION, ACTIVATION, Adult, Allergens, Antibodies, Neutralizing, B-Lymphocytes, Regulatory, Biomarkers |
| جغرافية الموضوع: | United States |
| الوصف: | BACKGROUND: Grass pollen subcutaneous immunotherapy (SCIT) is associated with induction of serum IgG4-associated inhibitory antibodies that prevent IgE-facilitated allergen binding to B cells. OBJECTIVE: To determine whether SCIT induces nasal allergen-specific IgG4 antibodies with inhibitory activity that correlate closely with clinical response. METHODS: In a cross-sectional, controlled study, nasal fluid and sera were collected during the grass pollen season from 10 SCIT-treated patients, 13 untreated allergics (SAR) and 12 non-atopic controls (NA). Nasal and serum IgE and IgG4 to Phleum pratense (Phl p) components were measured by ISAC microarray. Inhibitory activity was measured by IgE-FAB assay. IL-10+Breg cells were quantified in peripheral blood by flow cytometry. RESULTS: Nasal and serum Phl p1 and Phl p5-specific IgE levels were elevated in SAR compared to NA (all, P < .001) and SCIT group. Nasal IgG4 levels were increased in SCIT compared to SAR group (P < .001) during the pollen season compared to out of season. IgG-associated inhibitory activity in nasal fluid and serum was significantly increased in SCIT compared to SAR group (both, P < .001). The magnitude of the inhibitory activity was 96% in the nasal fluid compared to 66% in serum and was reversed following depletion of IgG in nasal fluid (P = .03) and serum (P = .002). Both nasal fluid (r = -0.67, P = .0011) and serum (r = -0.59, P = .0097) blocking activity correlated global symptom improvement. IL-10+Breg cells were increased in season compared to out of season in SCIT group (P < .01). CONCLUSION: For the first time, we show that nasal IgG4-associated inhibitory activity correlate closely with the clinical response to allergen immunotherapy in allergic rhinitis with/without asthma. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0091-6749 |
| العلاقة: | Journal of Allergy and Clinical Immunology; http://hdl.handle.net/10044/1/65397Test; G0601303; G1000758 |
| DOI: | 10.1016/j.jaci.2018.09.039 |
| الإتاحة: | https://doi.org/10.1016/j.jaci.2018.09.039Test http://hdl.handle.net/10044/1/65397Test |
| حقوق: | © 2018 Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma & Immunology. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.ECEED357 |
| قاعدة البيانات: | BASE |
| تدمد: | 00916749 |
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| DOI: | 10.1016/j.jaci.2018.09.039 |