دورية أكاديمية
Cell-free DNA methylome analysis for early preeclampsia prediction
| العنوان: | Cell-free DNA methylome analysis for early preeclampsia prediction |
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| المؤلفون: | De Borre, Marie, Che, Huiwen, Yu, Qian, Lannoo, Lore, De Ridder, Kobe, Vancoillie, Leen, DREESEN, Pauline, Van Den Ackerveken, Mika, Aerden, Mio, Galle, Eva, Breckpot, Jeroen, Van Keirsbilck, Joachim, GYSELAERS, Wilfried, Devriendt, Koen, Vermeesch, Joris Robert, Van Calsteren, Kristel, Thienpont, Bernard |
| المساهمون: | Lannoo, Lore/0000-0002-6271-221X, Thienpont, Bernard/0000-0002-8772-6845, Aerden, Mio/0000-0001-5044-2120, Dreesen, Pauline/0000-0003-4165-6121, De Ridder, Kobe/0000-0001-7343-8642, Vermeesch, Joris/0000-0002-3071-1191, Galle, Eva/0000-0001-9276-2552, Che, Huiwen/0000-0002-3651-069X, De Borre, Marie, Che, Huiwen, Yu, Qian, Lannoo, Lore, De Ridder, Kobe, Vancoillie, Leen, DREESEN, Pauline, Van Den Ackerveken, Mika, Aerden, Mio, Galle, Eva, Breckpot, Jeroen, Van Keirsbilck, Joachim, GYSELAERS, Wilfried, Devriendt, Koen, Vermeesch, Joris Robert, Van Calsteren, Kristel, Thienpont, Bernard |
| بيانات النشر: | NATURE PORTFOLIO |
| سنة النشر: | 2023 |
| المجموعة: | Document Server@UHasselt (Universiteit Hasselt) |
| مصطلحات موضوعية: | Pregnancy, Humans, Female, Epigenome, Area Under Curve, DNA Methylation, Pre-Eclampsia, Cell-Free Nucleic Acids |
| الوصف: | Preeclampsia (PE) is a leading cause for peripartal morbidity, especially if developing early in gestation. To enable prophylaxis in the prevention of PE, pregnancies at risk of PE must be identified early-in the first trimester. To identify at-risk pregnancies we profiled methylomes of plasma-derived, cell-free DNA from 498 pregnant women, of whom about one-third developed early-onset PE. We detected DNA methylation differences between control and PE pregnancies that enabled risk stratification at PE diagnosis but also presymptomatically, at around 12 weeks of gestation (range 9-14 weeks). The first-trimester risk prediction model was validated in an external cohort collected from two centers (area under the curve (AUC) = 0.75) and integrated with routinely available maternal risk factors (AUC = 0.85). The combined risk score correctly predicted 72% of patients with early-onset PE at 80% specificity. These preliminary results suggest that cell-free DNA methylation profiling is a promising tool for presymptomatic PE risk assessment, and has the potential to improve treatment and follow-up in the obstetric clinic. ; We thank T. Van Brussel and D. Lambrechts (VIB, KU Leuven) for help with high-throughput sequencing; the Obstetrics and Gynaecology unit (UZ Leuven) for help with sampling blood and placental tissue; members of the Leuven Center for Human Genetics for cfDNA extraction, storage and supply; the Leuven Genomics Core for sequencing; and all women who agreed to participate. Computing was performed at the Vlaams Supercomputer Center. This study was supported by Research Foundation – Flanders (FWO), grant nos. 1524119 N (to B.T. and J.R.V.), S003422N (to J.R.V. and B.T.) and G0B4822N and G0C7519N (to B.T.); by KU Leuven Bijzonder Onderzoeksfonds, grant no. 3M180296 (to B.T.); and by a KU Leuven C1 grant (to J.R.V. and B.T.). M.D.B. and M.A. are supported by a Research Foundation – Flanders (FWO) fellowship and B.T. by a BOFZAP mandate. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | NATURE MEDICINE, 29 (9) , p. 2206 -2215; http://hdl.handle.net/1942/41486Test; 2215; 2206; 29; 001063746200001 |
| DOI: | 10.1038/s41591-023-02510-5 |
| الإتاحة: | https://doi.org/10.1038/s41591-023-02510-5Test http://hdl.handle.net/1942/41486Test |
| حقوق: | The Author(s), under exclusive licence to Springer Nature America, Inc. 2023 ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.6A7B8C8D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41591-023-02510-5 |
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