دورية أكاديمية
Varicella-Zoster Virus IE4 Protein Interacts with SR Proteins and Exports mRNAs through the TAP/NXF1 Pathway.
| العنوان: | Varicella-Zoster Virus IE4 Protein Interacts with SR Proteins and Exports mRNAs through the TAP/NXF1 Pathway. |
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| المؤلفون: | Ote, Isabelle, Vandevenne, Patricia, Bontems, Sébastien, Medina-Palazon, C., Manet, E., Piette, Jacques, Lebrun, Marielle, Sadzot-Delvaux, Catherine |
| المساهمون: | GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège |
| المصدر: | PLoS ONE, 4 (11), e7882 (2009-11-18) |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2009 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | VZV IE4, mRNA export, post-transcriptional regulation, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | peer reviewed ; Available data suggest that the Varicella-Zoster virus (VZV) IE4 protein acts as an important regulator on VZV and cellular genes expression and could exert its functions at post-transcriptional level. However, the molecular mechanisms supported by this protein are not yet fully characterized. In the present study, we have attempted to clarify this IE4-mediated gene regulation and identify some cellular partners of IE4. By yeast two-hybrid and immunoprecipitation analysis, we showed that IE4 interacts with three shuttling SR proteins, namely ASF/SF2, 9G8 and SRp20. We positioned the binding domain in the IE4 RbRc region and we showed that these interactions are not bridged by RNA. We demonstrated also that IE4 strongly interacts with the main SR protein kinase, SRPK1, and is phosphorylated in in vitro kinase assay on residue Ser-136 contained in the Rb domain. By Northwestern analysis, we showed that IE4 is able to bind RNA through its arginine-rich region and in immunoprecipitation experiments the presence of RNA stabilizes complexes containing IE4 and the cellular export factors TAP/NXF1 and Aly/REF since the interactions are RNase-sensitive. Finally, we determined that IE4 influences the export of reporter mRNAs and clearly showed, by TAP/NXF1 knockdown, that VZV infection requires the TAP/NXF1 export pathway to express some viral transcripts. We thus highlighted a new example of viral mRNA export factor and proposed a model of IE4-mediated viral mRNAs export. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | urn:issn:1932-6203; https://orbi.uliege.be/handle/2268/33834Test; info:hdl:2268/33834; https://orbi.uliege.be/bitstream/2268/33834/1/OTE%202009.pdfTest; scopus-id:2-s2.0-70849134258; info:pmid:19924249 |
| DOI: | 10.1371/journal.pone.0007882 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0007882Test https://orbi.uliege.be/handle/2268/33834Test https://orbi.uliege.be/bitstream/2268/33834/1/OTE%202009.pdfTest |
| حقوق: | open access ; http://purl.org/coar/access_right/c_abf2Test ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.FF189CAA |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0007882 |