دورية أكاديمية
Absence of COVID-19-associated changes in plasma coagulation proteins and pulmonary thrombosis in the ferret model
| العنوان: | Absence of COVID-19-associated changes in plasma coagulation proteins and pulmonary thrombosis in the ferret model |
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| المؤلفون: | Kreft, Iris C., Winiarczyk, Roy R.A., Tanis, Fric J., van der Zwaan, Carmen, Schmitz, Katharina S., Hoogendijk, Arie J., de Swart, Rik L., Moscona, Anne, Porotto, Matteo, Salvatori, Daniela C.F., de Vries, Rory D., de Maat, Moniek P.M., van den Biggelaar, Maartje, van Vlijmen, Bart J.M. |
| المصدر: | Kreft , I C , Winiarczyk , R R A , Dutch Covid-19 and Thrombosis Coalition (DCTC) , Tanis , F J , van der Zwaan , C , Schmitz , K S , Hoogendijk , A J , de Swart , R L , Moscona , A , Porotto , M , Salvatori , D C F , de Vries , R D , de Maat , M P M , van den Biggelaar , M & van Vlijmen , B J M 2022 , ' Absence of COVID-19-associated changes in plasma coagulation proteins and pulmonary thrombosis in .... |
| سنة النشر: | 2022 |
| الوصف: | Background: Many patients who are diagnosed with coronavirus disease 2019 (COVID-19) suffer from venous thromboembolic complications despite the use of stringent anticoagulant prophylaxis. Studies on the exact mechanism(s) underlying thrombosis in COVID-19 are limited as animal models commonly used to study venous thrombosis pathophysiology (i.e. rats and mice) are naturally not susceptible to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Ferrets are susceptible to SARS-CoV-2 infection, successfully used to study virus transmission, and have been previously used to study activation of coagulation and thrombosis during influenza virus infection. Objectives: This study aimed to explore the use of (heat-inactivated) plasma and lung material from SARS-CoV-2-inoculated ferrets studying COVID-19-associated changes in coagulation and thrombosis. Material and methods: Histology and longitudinal plasma profiling using mass spectrometry-based proteomics approach was performed. Results: Lungs of ferrets inoculated intranasally with SARS-CoV-2 demonstrated alveolar septa that were mildly expanded by macrophages, and diffuse interstitial histiocytic pneumonia. However, no macroscopical or microscopical evidence of vascular thrombosis in the lungs of SARS-CoV-2-inoculated ferrets was found. Longitudinal plasma profiling revealed minor differences in plasma protein profiles in SARS-CoV-2-inoculated ferrets up to 2 weeks post-infection. The majority of plasma coagulation factors were stable and demonstrated a low coefficient of variation. Conclusions: We conclude that while ferrets are an essential and well-suited animal model to study SARS-CoV-2 transmission, their use to study SARS-CoV-2-related changes relevant to thrombotic disease is limited. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1016/j.thromres.2021.12.015 |
| الإتاحة: | https://doi.org/10.1016/j.thromres.2021.12.015Test https://pure.eur.nl/en/publications/1d56ce97-dd2c-4bb3-acb8-04504769e453Test https://pure.eur.nl/ws/files/45348950/Absence_of_COVID_19_associated_changes_in_plasma_coagulation_proteins_and_pulmonary_thrombosis_in_the_ferret_model.pdfTest http://www.scopus.com/inward/record.url?scp=85121608331&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.834C1243 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.thromres.2021.12.015 |
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