Simultaneous quantification of arctigenin and its glucuronide conjugate in mouse plasma using ultra‐high performance liquid chromatography coupled to tandem mass spectrometry
| العنوان: | Simultaneous quantification of arctigenin and its glucuronide conjugate in mouse plasma using ultra‐high performance liquid chromatography coupled to tandem mass spectrometry |
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| المؤلفون: | Takuya Awazuhara, Yusuke Nukui, Airi Yoshida, Michiko Sato, Satoshi Kishino, Yosuke Suzuki, Keiko Ohno, Rumi Fujioka, Tomoka Terashima, Keita Watanabe, Katsuya Tsuchihara |
| المصدر: | Journal of Separation Science. 44:1299-1306 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Antioxidant, Dried fruit, medicine.medical_treatment, Filtration and Separation, Mass spectrometry, Tandem mass spectrometry, Lignans, Analytical Chemistry, Mice, 03 medical and health sciences, chemistry.chemical_compound, Glucuronides, 0302 clinical medicine, Pharmacokinetics, Tandem Mass Spectrometry, medicine, Animals, Furans, Chromatography, High Pressure Liquid, Arctigenin, 030304 developmental biology, Mice, Inbred BALB C, 0303 health sciences, Chromatography, Chemistry, 030220 oncology & carcinogenesis, Glucuronide, Conjugate |
| الوصف: | Arctigenin is a natural lignin and a main active component of Fructus arctii, the dried fruit of Arctium lappa. This compound was reported to have some biological activities such as anti-inflammatory, antioxidant, antiviral, renoprotective, and antitumor effects. Arctigenin is mainly metabolized to arctigenin-4'-O-glucuronide by UDP-glucuronosyltransferase. In this study, a simultaneous quantification method was established and validated for measuring arctigenin and arctigenin-4'-O-glucuronide in mouse plasma using ultra-high performance liquid chromatography with tandem mass spectrometry. The assay fulfilled the requirements of the United States Food and Drug Administration guideline for assay validation, with a lower limit of quantification of 2.00 ng/mL for arctigenin and 50.0 ng/mL for arctigenin-4'-O-glucuronide. The recovery rate and matrix effect ranged from 78.4 to 102.8% and 92.5 to 106.3%, respectively, for arctigenin, and 74.3 to 109.2% and 94.9 to 110.2% for arctigenin-4'-O-glucuronide. The method was applied to the measurement of plasma concentrations of arctigenin and arctigenin-4'-O-glucuronide in the plasma of mice after administration of arctigenin. All measured concentrations were within the calibration ranges. Our novel method may be useful to measure plasma arctigenin and arctigenin-4'-O-glucuronide concentrations, and contribute to evaluate the pharmacokinetics of arctigenin and arctigenin-4'-O-glucuronide in mice. |
| تدمد: | 1615-9314 1615-9306 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e22cc6743f398a3d55ffed8f5e4d091Test https://doi.org/10.1002/jssc.202001078Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....5e22cc6743f398a3d55ffed8f5e4d091 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16159314 16159306 |
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