دورية أكاديمية
Unravelling the Impact of Cyclic Mechanical Stretch in Keratoconus-A Transcriptomic Profiling Study
| العنوان: | Unravelling the Impact of Cyclic Mechanical Stretch in Keratoconus-A Transcriptomic Profiling Study |
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| المؤلفون: | Akoto, Theresa, Cai, Jingwen, Nicholas, Sarah, McCord, Hayden, Estes, Amy J., Xu, Hongyan, Karamichos, Dimitrios, Liu, Yutao |
| المصدر: | International Journal of Molecular Sciences |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | UNTHSC Scholarly Repository (University. of North Texas Health Science Center) |
| مصطلحات موضوعية: | RNA-Sequencing, TGFbeta1 treatment, cornea, cyclic mechanical stretch (CMS), keratoconus (KC), Humans, Keratoconus / metabolism, Transcriptome, Cells, Cultured, Cornea / metabolism, Collagen / metabolism |
| الوصف: | Biomechanical and molecular stresses may contribute to the pathogenesis of keratoconus (KC). We aimed to profile the transcriptomic changes in healthy primary human corneal (HCF) and KC-derived cells (HKC) combined with TGFbeta1 treatment and cyclic mechanical stretch (CMS), mimicking the pathophysiological condition in KC. HCFs (n = 4) and HKCs (n = 4) were cultured in flexible-bottom collagen-coated 6-well plates treated with 0, 5, and 10 ng/mL of TGFbeta1 with or without 15% CMS (1 cycle/s, 24 h) using a computer-controlled Flexcell FX-6000T Tension system. We used stranded total RNA-Seq to profile expression changes in 48 HCF/HKC samples (100 bp PE, 70-90 million reads per sample), followed by bioinformatics analysis using an established pipeline with Partek Flow software. A multi-factor ANOVA model, including KC, TGFbeta1 treatment, and CMS, was used to identify differentially expressed genes (DEGs, |fold change| >/= 1.5, FDR /= 10 in >/=1 sample) in HKCs (n = 24) vs. HCFs (n = 24) and those responsive to TGFbeta1 and/or CMS. PANTHER classification system and the DAVID bioinformatics resources were used to identify significantly enriched pathways (FDR |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| تدمد: | 1422-0067 |
| العلاقة: | https://doi.org/10.3390/ijms24087437Test; Akoto, T., Cai, J., Nicholas, S., McCord, H., Estes, A. J., Xu, H., Karamichos, D., & Liu, Y. (2023). Unravelling the Impact of Cyclic Mechanical Stretch in Keratoconus-A Transcriptomic Profiling Study. International journal of molecular sciences, 24(8), 7437. https://doi.org/10.3390/ijms24087437Test; https://hdl.handle.net/20.500.12503/32352Test; 24 |
| الإتاحة: | https://doi.org/20.500.12503/32352Test https://doi.org/10.3390/ijms24087437Test https://hdl.handle.net/20.500.12503/32352Test |
| حقوق: | Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0Test/ ; © 2023 by the authors. |
| رقم الانضمام: | edsbas.B8DB21E8 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 |
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