المؤلفون: Jeffrey L. Schloßhauer, Lena Tholen, Alexander Körner, Stefan Kubick, Sofia Chatzopoulou, Anja Hönow, Anne Zemella

المصدر: Synthetic and Systems Biotechnology, Vol 9, Iss 3, Pp 416-424 (2024)

مصطلحات موضوعية: Inducible expression, CHO cells, Cell-free protein synthesis, CRISPR, T7 RNA polymerase, eIF2, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405805X24000450Test; https://doaj.org/toc/2405-805XTest

الوصول الحر: https://doaj.org/article/135f28cf17b64878a5997cacbe96703bTest

المؤلفون: Wei He, Xinya Zhang, Yangxiaoyu Zou, Ji Li, Chong Wang, Yucai He, Qiuheng Jin, Jianren Ye

المصدر: Molecules, Vol 29, Iss 11, p 2461 (2024)

مصطلحات موضوعية: mRNA integrity, fragmented mRNA, transcription, T7 RNA polymerase, mutation, synthesis, Organic chemistry, QD241-441

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1420-3049/29/11/2461Test; https://doaj.org/toc/1420-3049Test

الوصول الحر: https://doaj.org/article/3ad8d79e8f474e1fa447d94890e792dcTest

المؤلفون: Schloßhauer, Jeffrey L., Tholen, Lena, Körner, Alexander, Kubick, Stefan, Chatzopoulou, Sofia, Hönow, Anja, Zemella, Anne

مصطلحات موضوعية: Inducible expression, CHO cells, Cell-free protein synthesis, CRISPR, T7 RNA polymerase, eIF2, Rosa26, 500 Naturwissenschaften und Mathematik570 Biowissenschaften, Biologie570 Biowissenschaften, Biologie

العلاقة: https://doi.org/10.1016/j.synbio.2024.03.011Test; https://dx.doi.org/10.1016/j.synbio.2024.03.011Test

الإتاحة: https://doi.org/10.17169/refubium-4365410.1016/j.synbio.2024.03.011Test
https://refubium.fu-berlin.de/handle/fub188/43944Test

المؤلفون: Younghyeon Kim, Daehan Nam, Eun Sung Lee, Seokjoon Kim, Byung Seok Cha, Ki Soo Park

المصدر: Biosensors, Vol 14, Iss 1, p 47 (2024)

مصطلحات موضوعية: biological network, protein, aptamer, CRISPR/Cas, T7 RNA polymerase, Biotechnology, TP248.13-248.65

العلاقة: https://www.mdpi.com/2079-6374/14/1/47Test; https://doaj.org/toc/2079-6374Test; https://doaj.org/article/66ce774c814c4d4b9ce9ee3d48d839eeTest

الإتاحة: https://doi.org/10.3390/bios14010047Test
https://doaj.org/article/66ce774c814c4d4b9ce9ee3d48d839eeTest

المؤلفون: Heng Xia, Bingbing Yu, Yixin Jiang, Rui Cheng, Xueling Lu, Hui Wu, Bin Zhu

المصدر: RNA Biology, Vol 19, Iss 1, Pp 1130-1142 (2022)

مصطلحات موضوعية: dsrna, sgrna, mrna medicine, t7 rna polymerase, in vitro transcription, Genetics, QH426-470

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1547-6286Test; https://doaj.org/toc/1555-8584Test

الوصول الحر: https://doaj.org/article/a0b4c153499749698a56c482a2043fdeTest

المؤلفون: Sousa, Maria I., Freire, Mara G., Silva, Francisca A. e, Pedro, Augusto Q.

مصطلحات موضوعية: mRNA nanomedicines, Ionic liquid, Integrated manufacturing processes, Thermoreversible aqueous biphasic system, In vitro transcription, T7 RNA polymerase

وصف الملف: application/pdf

الإتاحة: http://hdl.handle.net/10773/36347Test

المؤلفون: Costa, Maria I., Freire, Mara G., Silva, Francisca A. e, Pedro, Augusto Q.

مصطلحات موضوعية: mRNA, Ionic liquid, Integrated manufacturing processes, Thermoreversible aqueous biphasic system, In vitro transcription, T7 RNA polymerase

وصف الملف: application/pdf

الإتاحة: http://hdl.handle.net/10773/36345Test

المؤلفون: Wang, Wenya, Li, Yuwenbin, Wang, Yaqiong, Shi, Chen, Li, Chenmeng, Li, Qiang, Linhardt, Robert J

المصدر: Biotechnology Advances. 36(8)

مصطلحات موضوعية: Biochemistry and Cell Biology, Biological Sciences, Genetics, Bacteriophage T7, DNA, Viral, Genetic Engineering, Promoter Regions, Genetic, Synthetic Biology, Transcription, Genetic, T7 RNA polymerase, T7 promoter, Transcription system, Synthetic biology, Engineering, Technology, Biotechnology, Biological sciences

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/5j02w3bhTest

المؤلفون: Yongan Wang, Yanli Ji, Lin Sun, Zhifen Huang, Sheng Ye, Weimin Xuan

مصطلحات موضوعية: Biochemistry, Microbiology, Genetics, Molecular Biology, Immunology, Developmental Biology, Cancer, Mental Health, Infectious Diseases, Virology, Computational Biology, Biological Sciences not elsewhere classified, thus holding promise, t7 rna polymerase, less explored means, control biological processes, thereby sustains sirtuin, essential lysine located, t7rnap variant requires, vitro systems, synthetic biology, sirtuin expression, relevant research, maintain homeostasis, great significance, enzymatic activities, dependent transcription, dependent sirtuins, deacetylase activity, catalytic core

العلاقة: https://figshare.com/articles/journal_contribution/A_Sirtuin-Dependent_T7_RNA_Polymerase_Variant/24881561Test

الإتاحة: https://doi.org/10.1021/acssynbio.3c00607.s001Test
https://figshare.com/articles/journal_contribution/A_Sirtuin-Dependent_T7_RNA_Polymerase_Variant/24881561Test

المؤلفون: Purhonen, Janne, Hofer, Anders, Kallijärvi, Jukka

المساهمون: STEMM - Stem Cells and Metabolism Research Program, Clinicum

مصطلحات موضوعية: T7 rna-polymerase, Nucleotides, Promoter, Mechanism, Analogs, Kinase, Rat, 1182 Biochemistry, cell and molecular biology

وصف الملف: application/pdf

العلاقة: We thank Vilma Wanne and Diviya Upadhyay for technical assistance, and Nina Sipari and the Viikki metabolomics unit (Helsinki Institute of Life Science) for the efforts to quantify uridine nucleotides by UPLC-MS, and Prof. Vineta Fellman for critically reading the manuscript. The graphical abstract was created with BioRender.com.; Purhonen , J , Hofer , A & Kallijärvi , J 2023 , ' Quantification of all 12 canonical ribonucleotides by real-time fluorogenic in vitro transcription ' , Nucleic Acids Research . https://doi.org/10.1093/nar/gkad1091Test; ORCID: /0000-0003-3773-7025/work/150707418; d2782741-07ce-455d-925c-e25675718f07; http://hdl.handle.net/10138/568334Test; 001108541200001

الإتاحة: http://hdl.handle.net/10138/568334Test