Galanin inhibits caerulein-stimulated pancreatic amylase secretion via cholinergic nerves and insulin
| العنوان: | Galanin inhibits caerulein-stimulated pancreatic amylase secretion via cholinergic nerves and insulin |
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| المؤلفون: | Colin Carati, Surendra R. Jaya, Ann Schloithe, James Toouli, Gino T. P. Saccone, Charmaine M. Woods, Savio G. Barreto |
| المصدر: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 297:G333-G339 |
| بيانات النشر: | American Physiological Society, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Atropine, medicine.medical_specialty, Physiology, medicine.medical_treatment, Pancreatic amylase secretion, Neuropeptide, Galanin, Muscarinic Antagonists, Tetrodotoxin, Cholinergic Agonists, In Vitro Techniques, Biology, Hexamethonium, Mice, Physiology (medical), Internal medicine, Paracrine Communication, medicine, Animals, Insulin, Receptor, Pancreas, Pancreatic hormone, Dose-Response Relationship, Drug, Hepatology, Pancreatic Exocrine Secretion, Diazoxide, digestive, oral, and skin physiology, Gastroenterology, Receptor, Galanin, Type 2, Glucose, Endocrinology, Cholinergic Fibers, Mechanism of action, Amylases, Carbachol, medicine.symptom, Ceruletide, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | Pancreatic exocrine secretion is affected by galanin, but the mechanisms involved are unclear. We aimed to determine the effect and elucidate the mechanism of action of exogenous galanin on basal and stimulated pancreatic amylase secretion in vitro. The effect of galanin on basal-, carbachol-, and caerulein-stimulated amylase secretion from isolated murine pancreatic lobules was measured. Carbachol and caerulein concentration-response relationships were established. Lobules were coincubated with galanin (10−12M to 10−7M), carbachol (10−6M), or caerulein (10−10M). Lobules were preincubated with atropine (10−5M), tetrodotoxin (10−5M), hexamethonium (10−5M), or diazoxide (10−7M and 10−4M) for 30 min followed by incubation with caerulein (10−10M) alone or combined with galanin (10−12M). Amylase secretion was expressed as percent of total lobular amylase. Immunohistochemical studies used the antigen retrieval technique and antisera for galanin receptor (GALR) 1, 2, and 3. Carbachol and caerulein stimulated amylase secretion in a concentration-dependent manner with maximal responses of two- and 1.7-fold over control evoked at 10−6M and 10−10M, respectively. Galanin (10−12M) completely inhibited caerulein-stimulated amylase secretion but had no effect on carbachol-stimulated or basal secretion. Atropine and tetrodotoxin pretreatment abolished the caerulein-stimulated amylase secretion, whereas hexamethonium had no significant effect. Diazoxide significantly reduced caerulein-stimulated amylase secretion by ∼80%. Galanin did not affect caerulein-stimulated amylase secretion in the presence of hexamethonium or diazoxide. Glucose-stimulated amylase secretion was also inhibited by galanin. Immunohistochemistry revealed islet cells labeled for GALR2. These data suggest that galanin may modulate caerulein-stimulated amylase secretion by acting on cholinergic nerves and/or islet cells possibly via GALR2 to regulate insulin release. |
| تدمد: | 1522-1547 0193-1857 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e52d295b3182675f3822d11c087b7df2Test https://doi.org/10.1152/ajpgi.00078.2009Test |
| رقم الانضمام: | edsair.doi.dedup.....e52d295b3182675f3822d11c087b7df2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221547 01931857 |
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