Preparation and evaluation of reduction-responsive micelles based on disulfide-linked chondroitin sulfate A-tocopherol succinate for controlled antitumour drug release
| العنوان: | Preparation and evaluation of reduction-responsive micelles based on disulfide-linked chondroitin sulfate A-tocopherol succinate for controlled antitumour drug release |
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| المؤلفون: | Weidong Li, Qinglan Yuan, Lijia Meng, Xin Xie, Junzhi Li, Songjin Cai, Jingmou Yu, Ming He, Jian Ding |
| المصدر: | Case Reports in Nephrology |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lung Neoplasms, Polymers, alpha-Tocopherol, Mice, Nude, Pharmaceutical Science, Case Report, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Micelle, Drug Delivery Systems, Stomach Neoplasms, In vivo, Cell Line, Tumor, Neoplasms, medicine, Animals, Humans, Doxorubicin, Disulfides, Cytotoxicity, Micelles, Pharmacology, A549 cell, Drug Carriers, Mice, Inbred BALB C, Antibiotics, Antineoplastic, Chemistry, Chondroitin Sulfates, 021001 nanoscience & nanotechnology, Xenograft Model Antitumor Assays, Controlled release, 0104 chemical sciences, Drug Liberation, A549 Cells, Delayed-Action Preparations, Critical micelle concentration, Biophysics, Doxorubicin Hydrochloride, 0210 nano-technology, human activities, medicine.drug |
| الوصف: | Objectives The study was to construct reduction-responsive chondroitin sulfate A (CSA)-conjugated TOS (CST) micelles with disulfide bond linkage, which was used for controlled doxorubicin (DOX) release and improved drug efficacy in vivo. Methods CST and non-responsive CSA-conjugated TOS (CAT) were synthesized, and the chemical structure was confirmed by Fourier transform infrared (FTIR) spectroscopy, proton nuclear magnetic resonance (1H NMR) spectroscopy, fluorescence spectrophotometer and dynamic light scattering. Antitumour drug DOX was physically encapsulated into CST and CSA by dialysis method. Cell uptake of DOX-based formulations was investigated by confocal laser scanning microscopy. In vitro cytotoxicity was studied in A549 and AGS cells. Furthermore, antitumour activity was evaluated in A549-bearing mice. Key findings CST and CAT can form self-assembled micelles, and have low value of critical micelle concentration. Notably, DOX-containing CST (D-CST) micelles demonstrated reduction-triggered drug release in glutathione-containing media. Further, reduction-responsive uptake of D-CST was observed in A549 cells. In addition, D-CST induced stronger cytotoxicity (P < 0.05) than DOX-loaded CAT (D-CAT) against A549 and AGS cells. Moreover, D-CST exhibited significantly stronger antitumour activity in A549-bearing nude mice than doxorubicin hydrochloride and D-CAT. Conclusions The reduction-responsive CST micelles enhanced the DOX effect at tumour site and controlled drug release. |
| تدمد: | 2042-7158 0022-3573 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c975eb3fda6e904eb7cc7432b5c500a2Test https://doi.org/10.1093/jpp/rgab096Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c975eb3fda6e904eb7cc7432b5c500a2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20427158 00223573 |
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