التفاصيل البيبلوغرافية
| العنوان: |
DataSheet_1_Plasma EVs Display Antigen-Presenting Characteristics in Patients With Allergic Rhinitis and Promote Differentiation of Th2 Cells.docx |
| المؤلفون: |
Shu-Bin Fang (3811081), Zhi-Rou Zhou (11537692), Ya-Qi Peng (11537695), Xiao-Qing Liu (28848), Bi-Xin He (11537698), De-Hua Chen (4642303), Dong Chen (448284), Qing-Ling Fu (740038) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, allergic rhinitis, extracellular vesicles, Der p 1, antigen presentation, type 2 T helper cells |
| الوصف: |
Background Allergic rhinitis (AR) is characterized by IgE-mediated mucosa response after exposure to allergens. Extracellular vesicles (EVs) are nano-size vesicles containing biological cargos for intercellular communications. However, the role of plasma EVs in pathogenesis of AR remains largely unknown. Methods Plasma EVs from patients with AR were isolated, quantified, and characterized. The expression of Der p 1 and antigen-presenting molecules on EVs was determined by Western blot, flow cytometry, or ELISA. PKH26- and CFSE (carboxyfluorescein succinimidyl ester)-stained AR-EVs were used to determine the uptake of EVs by CD4 + T cells and their effects on CD4 + T cell proliferation, respectively. Results Plasma EVs in healthy control (HC) and AR patients were similar in the concentration of particles, expression for specific EV markers, and both had structural lipid bilayer. However, the levels of Der p 1 on plasma EVs from both mild and moderate-severe AR patients were significantly higher than that on HC. The levels of antigen-presenting molecules on plasma EVs were similar from three subjects. Moreover, levels of Der p 1 on EVs in plasma, but not nasal secretion, were significantly associated with the symptom score of AR patients and level of plasma IL-13. Additionally, plasma EVs from patients with AR promoted the development of Th2 cells, while no effect was found on CD4 + T-cell proliferation. Conclusions Plasma EVs derived from patients with AR exhibited antigen-presenting characteristics and promoted differentiation of Th2 cells, thus providing novel understanding of the pathogenesis of AR. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/dataset/DataSheet_1_Plasma_EVs_Display_Antigen-Presenting_Characteristics_in_Patients_With_Allergic_Rhinitis_and_Promote_Differentiation_of_Th2_Cells_docx/16768324Test |
| DOI: |
10.3389/fimmu.2021.710372.s001 |
| الإتاحة: |
https://doi.org/10.3389/fimmu.2021.710372.s001Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.AF2C4249 |
| قاعدة البيانات: |
BASE |
