Detection of Mycobacterium avium subsp. paratuberculosis in a sheep flock in Tuscany
| العنوان: | Detection of Mycobacterium avium subsp. paratuberculosis in a sheep flock in Tuscany |
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| المؤلفون: | Barbara Turchi, Filippo Fratini, Alessia Galiero, Domenico Cerri, Giuseppe Colombani, Roberta Nuvoloni |
| المصدر: | Tropical Animal Health and Production. 47:1567-1571 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Genotyping, Immunodiffusion, Veterinary medicine, Sheeps, Genotype, Culture, Population, Sheep Diseases, Paratuberculosis, Spleen, Biology, Sheeps, Mycobacterium avium subsp. paratuberculosis, Garfagnana district, Culture, Polymerase chain reaction, Genotyping, Garfagnana district, Polymerase Chain Reaction, law.invention, Feces, Food Animals, Cheese, Ileum, law, medicine, Animals, Mesenteric lymph nodes, education, Sheep, Domestic, Polymerase chain reaction, education.field_of_study, Sheep, food and beverages, medicine.disease, Sheep flock, Mycobacterium avium subsp. paratuberculosis, Milk, medicine.anatomical_structure, Italy, Animal Science and Zoology, Lymph Nodes, Lymph |
| الوصف: | Paratuberculosis is an infectious disease which affects ruminants. In this work, a sheep flock in Garfagnana district (Tuscany, Italy) was examined by agar gel immune-diffusion (AGID) tests, culture, and PCR from feces, milk, tissue samples, and cheeses. At the first AGID test, 7/280 (2.5 %) animals were positive. From these animals, feces and milk samples were collected: 4/7 feces (57.14 %) and 2/7 milk samples (28.57 %) were positive to culture and 7/7 (100 %) feces samples and 1/7 milk samples (14.28 %) were positive to PCR; 2/4 (50 %) cheeses ripened for 14 days and 1/3 (33.33 %) cheese ripened for 20 days were positive to PCR, from which no viable microorganisms were isolated. Then, the AGID-positive animals were slaughtered and tissue samples were taken from one sheep with PCR-positive feces and milk: the liver, intestine, mesenteric lymph nodes, but not the spleen and mammary lymph nodes were positive to culture; all these samples were instead PCR-positive. After 1 year, a second AGID survey was performed on the remaining animals: 6/244 (2.45 %) subjects were positive to this test. Data obtained revealed the presence of paratuberculosis in a sheep population in Garfagnana. |
| تدمد: | 1573-7438 0049-4747 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::807006cd6965a01735192eed836a8d57Test https://doi.org/10.1007/s11250-015-0899-zTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....807006cd6965a01735192eed836a8d57 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15737438 00494747 |
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