التفاصيل البيبلوغرافية
| العنوان: |
Fabrication of a Novel 3D Extrusion Bioink Containing Processed Human Articular Cartilage Matrix for Cartilage Tissue Engineering. |
| المؤلفون: |
Aitchison, Alexandra Hunter, Allen, Nicholas B., Shaffrey, Isabel R., O'Neill, Conor N., Abar, Bijan, Anastasio, Albert T., Adams, Samuel B. |
| المصدر: |
Bioengineering (Basel); Apr2024, Vol. 11 Issue 4, p329, 11p |
| مصطلحات موضوعية: |
ARTICULAR cartilage, CARTILAGE regeneration, TISSUE engineering, CARTILAGE, POLYVINYL alcohol, EXTRACELLULAR matrix, CELL anatomy |
| مستخلص: |
Cartilage damage presents a significant clinical challenge due to its intrinsic avascular nature which limits self-repair. Addressing this, our study focuses on an alginate-based bioink, integrating human articular cartilage, for cartilage tissue engineering. This novel bioink was formulated by encapsulating C20A4 human articular chondrocytes in sodium alginate, polyvinyl alcohol, gum arabic, and cartilage extracellular matrix powder sourced from allograft femoral condyle shavings. Using a 3D bioprinter, constructs were biofabricated and cross-linked, followed by culture in standard medium. Evaluations were conducted on cellular viability and gene expression at various stages. Results indicated that the printed constructs maintained a porous structure conducive to cell growth. Cellular viability was 87% post printing, which decreased to 76% after seven days, and significantly recovered to 86% by day 14. There was also a notable upregulation of chondrogenic genes, COL2A1 (p = 0.008) and SOX9 (p = 0.021), suggesting an enhancement in cartilage formation. This study concludes that the innovative bioink shows promise for cartilage regeneration, demonstrating substantial viability and gene expression conducive to repair and suggesting its potential for future therapeutic applications in cartilage repair. [ABSTRACT FROM AUTHOR] |
|
Copyright of Bioengineering (Basel) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder