دورية أكاديمية
Single cell RNA-seq reveals profound transcriptional similarity between Barrett’s oesophagus and oesophageal submucosal glands
| العنوان: | Single cell RNA-seq reveals profound transcriptional similarity between Barrett’s oesophagus and oesophageal submucosal glands |
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| المؤلفون: | Owen, Richard Peter, White, Michael Joseph, Severson, David Tyler, Braden, Barbara, Bailey, Adam, Goldin, Robert, Wang, Lai Mun, Ruiz-Puig, Carlos, Maynard, Nicholas David, Green, Angie, Piazza, Paolo, Buck, David, Middleton, Mark Ross, Ponting, Chris Paul, Schuster-Böckler, Benjamin, Lu, Xin |
| المصدر: | Nature Communications ; volume 9, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, Multidisciplinary |
| الوصف: | Barrett’s oesophagus is a precursor of oesophageal adenocarcinoma. In this common condition, squamous epithelium in the oesophagus is replaced by columnar epithelium in response to acid reflux. Barrett’s oesophagus is highly heterogeneous and its relationships to normal tissues are unclear. Here we investigate the cellular complexity of Barrett’s oesophagus and the upper gastrointestinal tract using RNA-sequencing of single cells from multiple biopsies from six patients with Barrett’s oesophagus and two patients without oesophageal pathology. We find that cell populations in Barrett’s oesophagus, marked by LEFTY1 and OLFM4 , exhibit a profound transcriptional overlap with oesophageal submucosal gland cells, but not with gastric or duodenal cells. Additionally, SPINK4 and ITLN1 mark cells that precede morphologically identifiable goblet cells in colon and Barrett’s oesophagus, potentially aiding the identification of metaplasia. Our findings reveal striking transcriptional relationships between normal tissue populations and cells in a premalignant condition, with implications for clinical practice. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-018-06796-9 |
| الإتاحة: | https://doi.org/10.1038/s41467-018-06796-9Test https://www.nature.com/articles/s41467-018-06796-9.pdfTest https://www.nature.com/articles/s41467-018-06796-9Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.83CEF39E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-018-06796-9 |
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