دورية أكاديمية
iTRAQ-based mass spectrometry screen to identify serum biomarkers in systemic lupus erythematosus
| العنوان: | iTRAQ-based mass spectrometry screen to identify serum biomarkers in systemic lupus erythematosus |
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| المؤلفون: | Ting Zhang, Kamala Vanarsa, Ramesh Saxena, Chandra Mohan, C C Mok, Jack Hutcheson, Sneha Ravi Kumar, Satyavani Nukala, Haleigh Inthavong, Bruce Stanley, Tianfu Wu |
| المصدر: | Lupus Science and Medicine, Vol 11, Iss 1 (2024) |
| بيانات النشر: | BMJ Publishing Group, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: | Immunologic diseases. Allergy, RC581-607 |
| الوصف: | Objective Systemic lupus erythematosus (SLE) is a complex systemic autoimmune disorder with no reliable serum biomarkers currently available other than autoantibodies.Methods In the present study, isobaric tags for relative and absolute quantitation-based mass spectrometry was used to screen the sera of patients with SLE to uncover potential disease biomarkers.Results 85 common proteins were identified, with 16 being elevated (≥1.3) and 23 being decreased (≤0.7) in SLE. Of the 16 elevated proteins, serum alpha-1-microglobulin/bikunin precursor (AMBP), zinc alpha-2 glycoprotein (AZGP) and retinol-binding protein 4 (RBP4) were validated in independent cross-sectional cohorts (Cohort I, N=52; Cohort II, N=117) using an orthogonal platform, ELISA. Serum AMBP, AZGP and RBP4 were validated to be significantly elevated in both patients with inactive SLE and patients with active SLE compared with healthy controls (HCs) (p2.5) in Cohort I. All three proteins exhibited good discriminatory power for distinguishing active SLE and inactive SLE (area under the curve=0.82–0.96), from HCs. Serum AMBP exhibited the largest fold change in active SLE (5.96) compared with HCs and correlated with renal disease activity. The elevation in serum AMBP was validated in a second cohort of patients with SLE of different ethnic origins, correlating with serum creatinine (r=0.60, p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2022-0006 2053-8790 |
| العلاقة: | https://lupus.bmj.com/content/11/1/e000673.fullTest; https://doaj.org/toc/2053-8790Test |
| DOI: | 10.1136/lupus-2022-000673 |
| الوصول الحر: | https://doaj.org/article/11795427f92d4f60b99c4b0f5328a05aTest |
| رقم الانضمام: | edsdoj.11795427f92d4f60b99c4b0f5328a05a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20220006 20538790 |
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| DOI: | 10.1136/lupus-2022-000673 |