دورية أكاديمية
Transplantation: basic science and immune-tolerance
| العنوان: | Transplantation: basic science and immune-tolerance |
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| المؤلفون: | Sugawara, Mizuho, Ichimura, Shigetoshi, Kokubo, Kenichi, Shimbo, Toshihiro, Hirose, Minoru, Kobayashi, Hirosuke, Hribova, Petra, Brabcova, Irena, Honsova, Eva, Viklicky, Ondrej, Kute, Vivek B., Shah, Pankaj R., Vanikar, Aruna V., Gumber, Manoj R., Patel, Himanshu V., Modi, Pranjal R., Trivedi, Hargovind L., Trivedi, Varsha B., Nusrath, Syed, Minz, Mukut, Walker Minz, Ranjana, Sharma, Ashish, Singh, Sarabpreet, Jha, Vivekandanda, Joshi, Kusum, Richter, Rudolf, Köhler, Stefanie, Qidan, Sara, Scheuermann, Ernst, Kachel, Heinz-Georg, Gossmann, Jan, Gauer, Stefan, Seifried, Erhard, Geiger, Helmut, Seidl, Christian, Hauser, Ingeborg A., Hanssen, Lydia, Frye, Björn, Ostendorf, Tammo, Alidousty, Christina, Djudjaj, Sonja, Boor, Peter, Rauen, Thomas, Floege, Jürgen, Mertens, Peter, Raffetseder, Ute, García-Cenador, Begoña, Lopez-Novoa, Jose M., Iñiguez, Maria, Fernandez, Victor, Perez de Obanos, Pilar, Ruiz, Juan, Sanz-Gimenez, Juan R., Lopez-Marcos, Joaquin F., García-Criado, Javier, Van Craenenbroeck, Amaryllis H., Anguille, Sébastien H., Jürgens, Angelika, Cools, Nathalie, Van Camp, Kirsten, Stein, Barbara, Nijs, Griet, Berneman, Zwi, Ieven, Margareta, Van Damme, Pierre, Van Tendeloo, Vigor, Verpooten, Gert A., Gohel, Kalpesh, Hegde, Umapati, Gang, Sishir, Rajapurkar, Mohan, Erdogmus, Siyar, Sengul, Sule, Kocak, Senem, Kurultak, Ilhan, Kutlay, Sim, Keven, Kenan, Erbay, Bulent, Erturk, Sehsuvar, Kimura, Shogo, Imura, Junko, Atsumi, Hirokatsu, Fujimoto, Keiji, Chikazawa, Yoshihiro, Nakagawa, Masaru, Hayama, Tomoyuki, Okuyama, Hiroshi, Yamaya, Hideki, Yokoyama, Hitoshi, Libetta, Carmelo, Canevari, Michele, Sepe, Vincenzo, Margiotta, Elisabetta, Meloni, Federica, Martinelli, Claudia, Borettaz, Ilaria, Esposito, Pasquale, Portalupi, Valentina, Morosini, Monica, Solari, Nadia, Dal Canton, Antonio, Rusai, Krisztina, Schmaderer, Christoph, Hermans, Rob, Lutz, Jens, Heemann, Uwe, Baumann, Marcus, Cantaluppi, Vincenzo, Tamagnone, Michela, Dellepiane, Sergio, Medica, Davide, Dolla, Caterina, Messina, Maria, Manzione, Ana Maria, Tognarelli, Giuliana, Ranghino, Andrea, Biancone, Luigi, Camussi, Giovanni, Segoloni, Giuseppe Paolo, Ozkurt, Sultan, Sahin, Garip, Degirmenci, Nevbahar, Temiz, Gokhan, Musmul, Ahmet, Birdane, Alparslan, Tek, Müjgan, Tekin, Neslihan, Akyüz, Fahrettin, Yalcin, Ahmet Ugur, Lopez-Valverde, Antonio |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2011 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Friday 24 June 2011 |
| الوصف: | INTRODUCTION AND AIMS: Recently, a novel tissue engineering approach using stem cells with a decellularized scaffold for reconstitution of a complex organ, such as the heart, kidneys, lungs and liver has been proposed. These studies achieved organ-specific differentiation after the scaffold was seeded ex vivo with stem cells. This approach is suitable for the reconstitution of complex organs like the kidney and also has an advantage that a large pool of donor organs which are unsuitable for transplantation can be used to provide the scaffold. However, these studies were performed with organs harvested from small animals. A modified decellularization technique would be required to prepare a larger organ scaffold. The aim of the present study was to develop a processing method which can make a scaffold from porcine whole kidney. METHODS: Porcine whole kidney was attached to an arterial cannula. First, we used the same decellularizing protocol used for the rat kidney in which an effluent fluid containing detergent was recirculated. However, using porcine kidney, sufficient removal of large amounts of protein and cell debris was not achieved with recirculated effluent fluid. We used a single-pass flow system and compared stepwise protocols using different concentrations of nonionic (Triton X-100, polyoxyethylene octyl phenyl ether) and ionic detergents (SDS, sodium dodecyl sulfate). The flow rate of detergent-containing perfusate was adjusted to 100 -10 ml/min in a way that the pressure applied to the kidney would be under 75 mmHg. We evaluated the perfusate flow resistance and the amount of protein removed. The resultant kidney scaffold was stained with hematoxylin and eosin (HE) to assess cell removal and preservation of the microarchitecture. RESULTS: In the first treatment step, we perfused 0.01% to 1% SDS or 3% Triton-100 saline solution for 6 hr. Using high-concentration SDS, flow resistance increased, which may destroy the microarchitecture of the kidney, whereas the use of low-concentration SDS resulted in ... |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://ckj.oxfordjournals.org/cgi/content/short/4/suppl_2/4.s2.42Test; http://dx.doi.org/10.1093/ndtplus/4.s2.42Test |
| DOI: | 10.1093/ndtplus/4.s2.42 |
| الإتاحة: | https://doi.org/10.1093/ndtplus/4.s2.42Test http://ckj.oxfordjournals.org/cgi/content/short/4/suppl_2/4.s2.42Test |
| حقوق: | Copyright (C) 2011, European Renal Association - European Dialysis and Transplant Assoc |
| رقم الانضمام: | edsbas.3073ADC1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/ndtplus/4.s2.42 |
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