دورية أكاديمية
Radiological, biomechanical and histopathological characteristics of rat brain in Kaolin-induced hydrocephalus
| العنوان: | Radiological, biomechanical and histopathological characteristics of rat brain in Kaolin-induced hydrocephalus |
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| المؤلفون: | Samanci, M. Yavuz, Celik, Suat Erol, Coskun, Zafer Unsal, Ozcan, Deniz |
| المصدر: | Romanian Neurosurgery; Vol. XXXIII, No. 4 (December 2019); 498-507 ; 2344-4959 ; 1220-8841 |
| بيانات النشر: | London Academic Publishing |
| سنة النشر: | 2019 |
| المجموعة: | London Academic Publishing Ltd.: Arts & Humanities Journals |
| مصطلحات موضوعية: | brain, hydrocephalus, kaolin, mechanical properties, viscoelasticity |
| الوصف: | Objective: A better understanding of the pathophysiology and underlying mechanisms of brain damage in hydrocephalus is vital in developing diagnostic, observational and treatment tools that will have an impact on hydrocephalus outcomes. In this study, we aimed to demonstrate the radiological, biomechanical and histopathological characteristics of rat brain tissue in an experimental hydrocephalus model. Materials and Methods: Thirty-six male Sprague-Dawley rats (21 days old, weighing between 150 and 200 grams) were used in this study. Animals were randomly assigned to control (n = 6), 1-week hydrocephalus (n = 10), 2-week hydrocephalus (n = 10) and 3-week hydrocephalus (n = 10) groups. Hydrocephalus was induced with cisternal kaolin injection and controls received sham injection. Magnetic resonance imaging was used to measure ventricle size and cortical thickness. Vital signs, cerebral blood flow (CBF), mechanical tests and brain histology were assessed. Results: Three rats in the hydrocephalus group died during the follow-up, yielding an overall mortality of 10% among animals from hydrocephalus groups. Ventricular width, cross-sectional area of the lateral ventricles, ventricular index and ventricle / brain area ratio progressively increased and cortical thickness progressively decreased following kaolin injection. CBF was significantly lower at baseline than at 1st, 2nd and 3rd week (p < 0.05, for all). ICP was significantly elevated in all hydrocephalic groups in comparison with controls. EIT that was calculated from the first load-unload indentation test showed a significant increase at 2nd week post-injection (p=0.0001), indicating increased intracranial stiffness. However, this significant difference disappeared at 3rd week (p=0.956). Quantitative immunohistochemistry showed that hydrocephalic brains demonstrated significantly less NeuN-positive cells and significantly higher IBA-1-positive microglia and glial fibrillary acidic protein positive astrocytes cells in the cortex. Discussion and Conclusion: ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://journals.lapub.co.uk/index.php/roneurosurgery/article/view/1286/1139Test; https://journals.lapub.co.uk/index.php/roneurosurgery/article/view/1286Test |
| DOI: | 10.33962/roneuro-2019-080 |
| الإتاحة: | https://doi.org/10.33962/roneuro-2019-080Test https://journals.lapub.co.uk/index.php/roneurosurgery/article/view/1286Test |
| رقم الانضمام: | edsbas.8BD07E25 |
| قاعدة البيانات: | BASE |
| DOI: | 10.33962/roneuro-2019-080 |
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