A Ranking-Based Meta-Analysis Reveals Let-7 Family as a Meta-Signature for Grade Classification in Breast Cancer
| العنوان: | A Ranking-Based Meta-Analysis Reveals Let-7 Family as a Meta-Signature for Grade Classification in Breast Cancer |
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| المؤلفون: | Bala Gur Dedeoglu, Isik G. Yulug, Alp Aydos, Tufan Bekmez, Yasemin Öztemur, Betül Bozkurt |
| المصدر: | PLoS ONE PLoS ONE, Vol 10, Iss 5, p e0126837 (2015) |
| بيانات النشر: | Public Library of Science, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | PRDM1 gene, Microarray, genetic association, TYMS gene, dendritic cell lysosome associated membrane protein, lcsh:Medicine, Bioinformatics, SLC16A3 gene, ME1 gene, Transcriptome, LAMP3 gene, SQSTM1 gene, RDH10 gene, LMNB2 gene, SAMSN1 gene, lcsh:Science, microRNA 30a 3p, Oligonucleotide Array Sequence Analysis, transforming growth factor beta, Multidisciplinary, microRNA, messenger RNA, mitogen activated protein kinase, apoptosis, microRNA 99a, gene expression regulation, minichromosome maintenance protein 2, biological marker, monocarboxylate transporter 4, unclassified drug, Gene Expression Regulation, Neoplastic, SEC24A gene, Meta-analysis, cancer grading, SOCS1 gene, cell cycle, IQGAP3 gene, Female, DNA microarray, down regulation, POLR2C gene, signal transduction, heme oxygenase 1, Research Article, Genetic Markers, PSAT1 gene, Concordance, TXNRD1 gene, microRNA let 7, Breast Neoplasms, Biology, Real-Time Polymerase Chain Reaction, Article, SOAT1 gene, reverse transcription polymerase chain reaction, TRIP13 gene, Breast cancer, breast cancer, medicine, thioredoxin reductase 1, Humans, microRNA 320, Computer Simulation, human, SLC25A13 gene, gene, microRNA 30a 5p, mammalian target of rapamycin, microRNA 7, STMN1 gene, microRNA let 7d, microRNA let 7e, POLA2 gene, microRNA let 7f, microRNA let 7g, B lymphocyte induced maturation protein 1, vasculotropin, microRNA let 7i, lcsh:R, SLC7A5 gene, TCF19 gene, SELL gene, medicine.disease, MCM2 gene, microRNA let 7a, microRNA let 7b, human tissue, microRNA let 7c, HMOX1 gene, MicroRNAs, suppressor of cytokine signaling 1, Gene chip analysis, lcsh:Q, SRM gene, upregulation |
| الوصف: | Breast cancer is one of the most important causes of cancer-related deaths worldwide in women. In addition to gene expression studies, the progressing work in the miRNA area including miRNA microarray studies, brings new aspects to the research on the cancer development and progression. Microarray technology has been widely used to find new biomarkers in research and many transcriptomic microarray studies are available in public databases. In this study, the breast cancer miRNA and mRNA microarray studies were collected according to the availability of their data and clinical information, and combined by a newly developed ranking-based meta-analysis approach to find out candidate miRNA biomarkers (meta-miRNAs) that classify breast cancers according to their grades and explain the relation between miRNAs and mRNAs. This approach provided meta-miRNAs specific to breast cancer grades, pointing out let-7 family members as grade classifiers. The qRTPCR studies performed with independent breast tumors confirmed the potential biomarker role of let-7 family members (meta-miRNAs). The concordance between the meta-mRNAs and miRNA target genes specific to tumor grade (common genes) supported the idea of mRNAs as miRNA targets. The pathway analysis results showed that most of the let-7 family miRNA targets, and also common genes, were significantly taking part in cancer-related pathways. The qRT-PCR studies, together with bioinformatic analyses, confirmed the results of meta-analysis approach, which is dynamic and allows combining datasets from different platforms. © 2015 Oztemur et al. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d9b2e646f82654defa29b32a7b5e6c9Test http://europepmc.org/articles/PMC4433233Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3d9b2e646f82654defa29b32a7b5e6c9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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