Validation of the bag‐mediated filtration system for environmental surveillance of poliovirus in Nairobi, Kenya
| العنوان: | Validation of the bag‐mediated filtration system for environmental surveillance of poliovirus in Nairobi, Kenya |
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| المؤلفون: | Angela Coulliette-Salmond, Benlick Mwangi, Christine S. Fagnant-Sperati, Everardo Vega, Fhatuwani B. Ngwana, Marianne Wolfaardt, Y Ren, R Nzunza, S Jeffries-Miles, A Chepkurui, John Scott Meschke, David S. Boyle, Alexandra L. Kossik, Evans Komen, Peter Borus, Jeffry H. Shirai, Maureen B. Taylor, Nicola K. Beck, Peter N. Matsapola, Nicolette A. Zhou, W.B. Van Zyl, James Nyangao, Joanne Hassan, Silvia Peñaranda, Cara C. Burns |
| المصدر: | Journal of Applied Microbiology |
| بيانات النشر: | Oxford University Press (OUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Veterinary medicine, environmental surveillance, Serogroup, medicine.disease_cause, Applied Microbiology and Biotechnology, 03 medical and health sciences, Public Health/Clinical Microbiology, medicine, Humans, Diagnostic screening, wastewater, 030304 developmental biology, filtration, 0303 health sciences, poliovirus, Sewage, 030306 microbiology, business.industry, Environmental surveillance, Poliovirus, ViroCap, Oral polio vaccine, Original Articles, General Medicine, Kenya, enteric viruses, Poliovirus Vaccine, Oral, Separation method, Original Article, business, Environmental Monitoring, Poliomyelitis, Biotechnology |
| الوصف: | Aims This study compared the bag‐mediated filtration system (BMFS) and standard WHO two‐phase separation methods for poliovirus (PV) environmental surveillance, examined factors impacting PV detection and monitored Sabin‐like (SL) PV type 2 presence with withdrawal of oral polio vaccine type 2 (OPV2) in April 2016. Methods and Results Environmental samples were collected in Nairobi, Kenya (Sept 2015–Feb 2017), concentrated via BMFS and two‐phase separation methods, then assayed using the WHO PV isolation algorithm and intratypic differentiation diagnostic screening kit. SL1, SL2 and SL3 were detected at higher rates in BMFS than two‐phase samples (P 0·05), while SL3 was detected less frequently with higher shipment temperatures (P = 0·027). SL2 was detected more frequently before OPV2 withdrawal in BMFS and two‐phase samples (P |
| تدمد: | 1365-2672 1364-5072 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4db502b67b4c743ddb0553dc3af64655Test https://doi.org/10.1111/jam.14807Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4db502b67b4c743ddb0553dc3af64655 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652672 13645072 |
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