دورية أكاديمية
CpG and Interleukin-15 Synergize to Enhance IFN-gamma Production by Activated CD8(+) T Cells
| العنوان: | CpG and Interleukin-15 Synergize to Enhance IFN-gamma Production by Activated CD8(+) T Cells |
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| المؤلفون: | Cobb, Dustin, Guo, Sigi, Smeltz, Ronald B. |
| المصدر: | Bioelectrics Publications |
| بيانات النشر: | ODU Digital Commons |
| سنة النشر: | 2013 |
| المجموعة: | Old Dominion University: ODU Digital Commons |
| مصطلحات موضوعية: | Rypanosoma cruzi infection, Bacterial DNA, Chagas disease, Dendritic cells, Effector cells, Vaccine, Cytokines, Biomedical Engineering and Bioengineering, Microbiology |
| الوصف: | Interleukin-15 (IL-15) regulates the development and maintenance of memory CD8(+) T cells. Paradoxically, we previously reported that IL-15 could enhance CD8(+) T-cell responses to IL-12, a proinflammatory cytokine required for optimal priming of effector CD8(+) T cells. To expand the physiological relevance of these findings, we tested IL-15 for its ability to enhance T-cell responses to bacterial CpG. Expectedly, CpG enhanced the production of IFN-gamma by CD8(+) T cells polyclonally activated with anti-CD3. However, addition of IL-15 to CpG-stimulated cultures led to a striking increase in IFN-gamma production. The effect of CpG and IL-15 was also evident with CD8(+) T cells recovered from mice infected with the parasite Trypanosoma cruzi (T. cruzi) and restimulated with antigen. The observed synergy between CpG and IL-15 occurred in an IL-12-dependent manner, and this effect could even be demonstrated in cocultures of activated CD8(+) T cells and CD4(+)CD25(+) regulatory T cells. Although IFN-gamma was not essential for CpG-induced IL-12, the ability of CpG and IL-15 to act on CD8(+) T cells required expression of the IFN-gamma-inducible transcription factor T-bet. These data have important implications for development of vaccines and design of therapies to boost CD8(+) T-cell responses to infectious agents and tumors. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.odu.edu/bioelectrics_pubs/47Test; https://digitalcommons.odu.edu/context/bioelectrics_pubs/article/1046/viewcontent/Cobb_2013_CpG_and_Interleukin_.pdfTest |
| DOI: | 10.1155/2013/924023 |
| الإتاحة: | https://doi.org/10.1155/2013/924023Test https://digitalcommons.odu.edu/bioelectrics_pubs/47Test https://digitalcommons.odu.edu/context/bioelectrics_pubs/article/1046/viewcontent/Cobb_2013_CpG_and_Interleukin_.pdfTest |
| رقم الانضمام: | edsbas.377CA423 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1155/2013/924023 |
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