دورية أكاديمية
Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
| العنوان: | Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals |
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| المؤلفون: | Leire Egia-Mendikute 1,8, Alexandre Bosch 1,8, Endika Prieto-Fernández 1, So Young Lee1, Borja Jiménez-Lasheras1, Ana García del Río1, Asier Antoñana-Vildosola1, Chiara Bruzzone2, Maider Bizkarguenaga2, Nieves Embade2, Rubén Gil-Redondo 2, María Luz Martínez-Chantar3,4, Marcos López-Hoyos5, Nicola G. A. Abrescia 4,6,7, José M. Mato 2,4, Óscar Millet2, Asís Palazón 1,7✉ |
| سنة النشر: | 2021 |
| المجموعة: | Zenodo |
| الوصف: | There is an ongoing need of developing sensitive and specific methods for the determination of SARS-CoV-2 seroconversion. For this purpose, we have developed a multiplexed flow cytometric bead array (C19BA) that allows the identification of IgG and IgM antibodies against three immunogenic proteins simultaneously: the spike receptor-binding domain (RBD), the spike protein subunit 1 (S1) and the nucleoprotein (N). Using different cohorts of samples collected before and after the pandemic, we show that this assay is more sensitive than ELISAs performed in our laboratory. The combination of three viral antigens allows for the interrogation of full seroconversion. Importantly, we have detected N-reactive antibodies in COVID-19-negative individuals. Here we present an immunoassay that can be easily implemented and has superior potential to detect low antibody titers compared to current gold standard serology methods |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://zenodo.org/record/5533978Test; https://doi.org/10.5281/zenodo.5533978Test; oai:zenodo.org:5533978 |
| DOI: | 10.5281/zenodo.5533978 |
| الإتاحة: | https://doi.org/10.5281/zenodo.5533978Test https://doi.org/10.5281/zenodo.5533977Test https://zenodo.org/record/5533978Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/4.0/legalcodeTest |
| رقم الانضمام: | edsbas.691F4E45 |
| قاعدة البيانات: | BASE |
| DOI: | 10.5281/zenodo.5533978 |
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