Activation of the Pseudomonas aeruginosa Type III Secretion System Requires an Intact Pyruvate Dehydrogenase aceAB Operon
| العنوان: | Activation of the Pseudomonas aeruginosa Type III Secretion System Requires an Intact Pyruvate Dehydrogenase aceAB Operon |
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| المؤلفون: | Benoît Polack, Bertrand Toussaint, Rozen Leberre, Olivier Epaulard, Benoit Guery, Arjan de Groot, Ina Attree, Denis Dacheux |
| المصدر: | Infection and Immunity. 70:3973-3977 |
| بيانات النشر: | American Society for Microbiology, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Pore Forming Cytotoxic Proteins, Cystic Fibrosis, Histidine Kinase, Neutrophils, Operon, Immunology, Mutant, Pyruvate Dehydrogenase Complex, Dihydrolipoyllysine-Residue Acetyltransferase, medicine.disease_cause, Microbiology, Type three secretion system, Rats, Sprague-Dawley, Bacterial Proteins, Phosphoprotein Phosphatases, Pneumonia, Bacterial, medicine, Animals, Humans, Pseudomonas Infections, Secretion, Cellular Microbiology: Pathogen-Host Cell Molecular Interactions, L-Lactate Dehydrogenase, biology, Pseudomonas aeruginosa, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Pyruvate dehydrogenase complex, Molecular biology, Rats, DNA-Binding Proteins, Disease Models, Animal, Mutagenesis, Insertional, Infectious Diseases, Pseudomonadales, Trans-Activators, Parasitology, Protein Kinases, Pseudomonadaceae |
| الوصف: | Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn 5 Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity. |
| تدمد: | 1098-5522 0019-9567 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef4ac8c2a06794f5c7364020b1618b0cTest https://doi.org/10.1128/iai.70.7.3973-3977.2002Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ef4ac8c2a06794f5c7364020b1618b0c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
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