Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG
| العنوان: | Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG |
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| المؤلفون: | Johanna Martins-Luna, Naysha Mallqui Espinoza, Erika Misaico, Adriana Viñas-Ospino, Ronald A. Aquino Ortega, Wilmer Silva-Caso, Walter Espinoza Espíritu, Juana del Valle-Mendoza, Luis J. del Valle, Claudia Weilg, Carlos Palomares-Reyes, Miguel Angel Aguilar-Luis, Luciana Stimmler |
| المساهمون: | Universitat Politècnica de Catalunya. Departament d'Enginyeria Química, Universitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables., Universitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables, juana.delvalle@upc.pe |
| المصدر: | International Journal of Infectious Diseases, Vol 81, Iss, Pp 31-37 (2019) Recercat. Dipósit de la Recerca de Catalunya instname UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) CONCYTEC-Institucional Consejo Nacional de Ciencia Tecnología e Innovación Tecnológica instacron:CONCYTEC Universidad Peruana de Ciencias Aplicadas (UPC) Repositorio Academico-UPC UPC-Institucional Universidad Peruana de Ciencias Aplicadas instacron:UPC International Journal of Infectious Diseases |
| بيانات النشر: | Elsevier, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, myalgia, age distribution, viruses, Viral Nonstructural Proteins, Dengue virus, Antibodies, Viral, medicine.disease_cause, Dengue fever, Serology, Dengue, immunoglobulin G, Perú, 0302 clinical medicine, secondary infection, Peru, Diagnosis, immunoglobulin M, 030212 general & internal medicine, comparative study, fever, Arbovirus, Reverse Transcriptase Polymerase Chain Reaction, Transmission (medicine), adult, virus diseases, General Medicine, Middle Aged, virus transmission, Infectious Diseases, Real-time polymerase chain reaction, female, Female, diagnostic accuracy, medicine.symptom, headache, Adult, Microbiology (medical), Adolescent, Fever, 030106 microbiology, Enzyme-Linked Immunosorbent Assay, Serogroup, Virus, Article, lcsh:Infectious and parasitic diseases, reverse transcription polymerase chain reaction, Young Adult, 03 medical and health sciences, odynophagia, Enginyeria química [Àrees temàtiques de la UPC], male, medicine, Humans, Serologic Tests, nonstructural protein 1, controlled study, lcsh:RC109-216, diagnostic test accuracy study, human, arthralgia, DENV, Dengue Virus, biochemical phenomena, metabolism, and nutrition, medicine.disease, Virology, major clinical study, RNA extraction, enzyme linked immunosorbent assay, Immunoglobulin M, sensitivity and specificity, Immunoglobulin G |
| الوصف: | This work was supported by Cienciativa of CONCYTEC Peru, under contract number 164-2016-FONDECYT, and the Programa Nacional de Innovación para la Competitividad y Productividad (Innóvate Perú), under contract number 116-PNICP-PIAP-2015. Background: Huánuco is a central eastern region of Peru whose geography includes high forest and low jungle, as well as a mountain range that constitutes the inter-Andean valleys. It is considered a region endemic for dengue due to the many favorable conditions that facilitate transmission of the virus. Methods: A total of 268 serum samples from patients in Huánuco, Peru with an acute febrile illness were assessed for the presence of dengue virus (DENV) via RT-PCR and NS1, IgM, and IgG ELISA during December 2015 and March 2016. Results: DENV was detected in 25% of samples via RT-PCR, 19% of samples by NS1 antigen ELISA, and 10.5% of samples by IgM ELISA. DENV IgG was detected in 15.7% of samples by ELISA. The most frequent symptoms associated with fever across all groups were headache, myalgia, and arthralgia, with no significant difference between the four test methods Conclusions: In this study, DENV was identified in up to 25% of the samples using the standard laboratory method. In addition, a correlation was established between the frequency of positive results and the serological tests that determine NS1, IgM, and IgG. There is an increasing need for point-of-care tests to strengthen epidemiological surveillance in Peru. Revisión por pares Revisión por pares |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1201-9712 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95d06733aefba89096cfe1133887b2f7Test http://www.sciencedirect.com/science/article/pii/S1201971219300335Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....95d06733aefba89096cfe1133887b2f7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 12019712 |
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