التفاصيل البيبلوغرافية
العنوان:
Representative Sequencing: Unbiased Sampling of Solid Tumor Tissue
المؤلفون:
Kevin Litchfield , Stacey Stanislaw , Lavinia Spain , Lisa L. Gallegos , Andrew Rowan , Desiree Schnidrig , Heidi Rosenbaum , Alexandre Harle , Lewis Au , Samantha M. Hill , Zayd Tippu , Jennifer Thomas , Lisa Thompson , Hang Xu , Stuart Horswell , Aoune Barhoumi , Carol Jones , Katherine F. Leith , Daniel L. Burgess , Thomas B.K. Watkins , Emilia Lim , Nicolai J. Birkbak , Philippe Lamy , Iver Nordentoft , Lars Dyrskjøt , Lisa Pickering , Stephen Hazell , Mariam Jamal-Hanjani , James Larkin , Charles Swanton , Nelson R. Alexander , Samra Turajlic , Chris Abbosh , Kai-Keen Shiu , John Bridgewater , Daniel Hochhauser , Martin Forster , Siow-Ming Lee , Tanya Ahmad , Dionysis Papadatos-Pastos , Sam Janes , Peter Van Loo , Katey Enfield , Nicholas McGranahan , Ariana Huebner , Sergio Quezada , Stephan Beck , Peter Parker , Henning Walczak , Tariq Enver , Rob Hynds , Mary Falzon , Ian Proctor , Ron Sinclair , Chi-wah Lok , Zoe Rhodes , David Moore , Teresa Marafioti , Elaine Borg , Miriam Mitchison , Reena Khiroya , Giorgia Trevisan , Peter Ellery , Mark Linch , Sebastian Brandner , Crispin Hiley , Selvaraju Veeriah , Maryam Razaq , Heather Shaw , Gert Attard , Mita Afroza Akther , Cristina Naceur-Lombardelli , Lizi Manzano , Maise Al-Bakir , Simranpreet Summan , Nnenna Kanu , Sophie Ward , Uzma Asghar , Faye Gishen , Adrian Tookman , Paddy Stone , Caroline Stirling , Andrew Furness , Kim Edmonds , Nikki Hunter , Sarah Sarker , Sarah Vaughan , Mary Mangwende , Karla Pearce , Scott Shepherd , Haixi Yan , Ben Shum , Eleanor Carlyle , Steve Hazell , Annika Fendler , Fiona Byrne , Nadia Yousaf , Sanjay Popat , Olivia Curtis , Gordon Stamp , Antonia Toncheva , Emma Nye , Aida Murra , Justine Korteweg , Nahid Sheikh , Debra Josephs , Ashish Chandra , James Spicer , Ula Mahadeva , Anna Green , Ruby Stewart , Lara-Rose Iredale , Tina Mackay , Ben Deakin , Debra Enting , Sarah Rudman , Sharmistha Ghosh , Lena Karapagniotou , Elias Pintus , Andrew Tutt , Sarah Howlett , Vasiliki Michalarea , James Brenton , Carlos Caldas , Rebecca Fitzgerald , Merche Jimenez-Linan , Elena Provenzano , Alison Cluroe , Grant Stewart , Colin Watts , Richard Gilbertson , Ultan McDermott , Simon Tavare , Emma Beddowes , Patricia Roxburgh , Andrew Biankin , Anthony Chalmers , Sioban Fraser , Karin Oien , Andrew Kidd , Kevin Blyth , Matt Krebs , Fiona Blackhall , Yvonne Summers , Caroline Dive , Richard Marais , Fabio Gomes , Mat Carter , Jo Dransfield , John Le Quesne , Dean Fennell , Jacqui Shaw , Babu Naidu , Shobhit Baijal , Bruce Tanchel , Gerald Langman , Andrew Robinson , Martin Collard , Peter Cockcroft , Charlotte Ferris , Hollie Bancroft , Amy Kerr , Gary Middleton , Joanne Webb , Salma Kadiri , Peter Colloby , Bernard Olisemeke , Rodelaine Wilson , Ian Tomlinson , Sanjay Jogai , Christian Ottensmeier , David Harrison , Massimo Loda , Adrienne Flanagan , Mairead McKenzie , Allan Hackshaw , Jonathan Ledermann , Abby Sharp , Laura Farrelly , Hayley Bridger
المصدر:
Cell Reports, Vol 31, Iss 5, Pp - (2020)
بيانات النشر:
Elsevier, 2020.
سنة النشر:
2020
المجموعة:
LCC:Biology (General)
مصطلحات موضوعية:
tumor sampling , tumor sequencing , representative sampling , molecular profiling , tumor hetereogeneity , biomarkers , Biology (General) , QH301-705.5
الوصف:
Summary: Although thousands of solid tumors have been sequenced to date, a fundamental under-sampling bias is inherent in current methodologies. This is caused by a tissue sample input of fixed dimensions (e.g., 6 mm biopsy), which becomes grossly under-powered as tumor volume scales. Here, we demonstrate representative sequencing (Rep-Seq) as a new method to achieve unbiased tumor tissue sampling. Rep-Seq uses fixed residual tumor material, which is homogenized and subjected to next-generation sequencing. Analysis of intratumor tumor mutation burden (TMB) variability shows a high level of misclassification using current single-biopsy methods, with 20% of lung and 52% of bladder tumors having at least one biopsy with high TMB but low clonal TMB overall. Misclassification rates by contrast are reduced to 2% (lung) and 4% (bladder) when a more representative sampling methodology is used. Rep-Seq offers an improved sampling protocol for tumor profiling, with significant potential for improved clinical utility and more accurate deconvolution of clonal structure.
نوع الوثيقة:
article
وصف الملف:
electronic resource
اللغة:
English
تدمد:
2211-1247
العلاقة:
http://www.sciencedirect.com/science/article/pii/S2211124720304605Test ; https://doaj.org/toc/2211-1247Test
DOI:
10.1016/j.celrep.2020.107550
الوصول الحر:
https://doaj.org/article/5542750c54464de39c1b869ec12a56ceTest
رقم الانضمام:
edsdoj.5542750c54464de39c1b869ec12a56ce
قاعدة البيانات:
Directory of Open Access Journals
Full Text Finder 