Expression and Distribution of Glucagon-Like Peptide-1 Receptor mRNA, Protein and Binding in the Male Nonhuman Primate (Macaca mulatta) Brain
| العنوان: | Expression and Distribution of Glucagon-Like Peptide-1 Receptor mRNA, Protein and Binding in the Male Nonhuman Primate (Macaca mulatta) Brain |
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| المؤلفون: | Melissa Kirigiti, Rikley Buckingham, Kevin L. Grove, Anna Secher, Lotte Bjerre Knudsen, Niels Vrang, Sarah Juel Paulsen, Kristy M. Heppner, Charles Pyke |
| المصدر: | Endocrinology. 156:255-267 |
| بيانات النشر: | The Endocrine Society, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, endocrine system, medicine.medical_specialty, Central nervous system, In situ hybridization, Macaque, Antibodies, Glucagon-Like Peptide-1 Receptor, Endocrinology, Antibody Specificity, Glucagon-Like Peptide 1, biology.animal, Internal medicine, Receptors, Glucagon, medicine, Animals, Tissue Distribution, RNA, Messenger, Receptor, In Situ Hybridization, biology, digestive, oral, and skin physiology, Area postrema, Solitary tract, Brain, Neuroendocrinology, Macaca mulatta, medicine.anatomical_structure, Dorsal motor nucleus, Gene Expression Regulation, Nucleus, hormones, hormone substitutes, and hormone antagonists, Protein Binding |
| الوصف: | Glucagon-like peptide-1 (GLP-1) is released from endocrine L-cells lining the gut in response to food ingestion. However, GLP-1 is also produced in the nucleus of the solitary tract, where it acts as an anorectic neurotransmitter and key regulator of many autonomic and neuroendocrine functions. The expression and projections of GLP-1-producing neurons is highly conserved between rodent and primate brain, although a few key differences have been identified. The GLP-1 receptor (GLP-1R) has been mapped in the rodent brain, but no studies have described the distribution of GLP-1Rs in the nonhuman primate central nervous system. Here, we characterized the distribution of GLP-1R mRNA and protein in the adult macaque brain using in situ hybridization, radioligand receptor autoradiography, and immunohistochemistry with a primate specific GLP-1R antibody. Immunohistochemistry demonstrated that the GLP-1R is localized to cell bodies and fiber terminals in a very selective distribution throughout the brain. Consistent with the functional role of the GLP-1R system, we find the highest concentration of GLP-1R-immunoreactivity present in select hypothalamic and brainstem regions that regulate feeding, including the paraventricular and arcuate hypothalamic nuclei, as well as the area postrema, nucleus of the solitary tract, and dorsal motor nucleus of the vagus. Together, our data demonstrate that GLP-1R distribution is highly conserved between rodent and primate, although a few key species differences were identified, including the amygdala, where GLP-1R expression is much higher in primate than in rodent. |
| تدمد: | 1945-7170 0013-7227 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c6c24a55edc4203b1e0e9f8bb131d0aTest https://doi.org/10.1210/en.2014-1675Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7c6c24a55edc4203b1e0e9f8bb131d0a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457170 00137227 |
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