دورية أكاديمية
The protease associated (PA) domain in ScpA from Streptococcus pyogenes plays a role in substrate recruitment
| العنوان: | The protease associated (PA) domain in ScpA from Streptococcus pyogenes plays a role in substrate recruitment |
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| المؤلفون: | Sophie McKenna, Frances Aylward, Xeni Miliara, Rikin J. Lau, Camilla Berg Huemer, Sean P. Giblin, Kristin K. Huse, Mingyang Liang, Lucy Reeves, Max Pearson, Yingqi Xu, Sarah L Rouse, James E. Pease, Shiranee Sriskandan, Todd Kagawa, JAKKI COONEY, Stephen Matthews |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Biological sciences, bacterial cell envelope protease, ScpA, solution NMR, group A Streptococcus, C5a and C3a |
| الوصف: | Annually, over 18 million disease cases and half a million deaths worldwide are estimated to be caused by Group A Streptococcus. ScpA (or C5a peptidase) is a well characterised member of the cell enveleope protease family, which possess a S8 subtilisin-like catalytic domain and a shared multi-domain architecture. ScpA cleaves complement factors C5a and C3a, impairing the function of these critical anaphylatoxins and disrupts complement-mediated innate immunity. Although the high resolution structure of ScpA is known, the details of how it recognises its substrate are only just emerging. Previous studies have identified a distant exosite on the 2nd fibronectin domain that plays an important role in recruitment via an interaction with the substrate core. Here, using a combination of solution NMR spectroscopy, mutagenesis with functional assays and computational approaches we identify a second exosite within the protease-associated (PA) domain. We propose a model in which the PA domain assists optimal delivery of the substrate’s C terminus to the active site for cleavage. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/The_protease_associated_PA_domain_in_ScpA_from_Streptococcus_pyogenes_plays_a_role_in_substrate_recruitment/24138828Test |
| DOI: | 10.34961/researchrepository-ul.24138828.v1 |
| الإتاحة: | https://doi.org/10.34961/researchrepository-ul.24138828.v1Test https://figshare.com/articles/journal_contribution/The_protease_associated_PA_domain_in_ScpA_from_Streptococcus_pyogenes_plays_a_role_in_substrate_recruitment/24138828Test |
| حقوق: | CC BY-NC-SA 4.0 |
| رقم الانضمام: | edsbas.A4485EA1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.34961/researchrepository-ul.24138828.v1 |
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