التفاصيل البيبلوغرافية
| العنوان: |
Multiplexed Magnetofluorescent Bioplatform for the Sensitive Detection of SARS-CoV‑2 Viral RNA without Nucleic Acid Amplification |
| المؤلفون: |
Riham Zayani (11217724), Dorra Rezig (11217727), Wasfi Fares (3875773), Mouna Marrakchi (11217730), Makram Essafi (267243), Noureddine Raouafi (1821886) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Medicine, Microbiology, Cell Biology, Molecular Biology, Biotechnology, Cancer, Infectious Diseases, Virology, Biological Sciences not elsewhere classified, non-polio viruses, ORF 1a, HRP, N genes, SARS-CoV -2 RNA, horseradish peroxidase, death toll, SARS-CoV -2 infection, RT-qPCR method, detection, West Nile, magnetofluorescent bioplatform, fluorescence response, SARS-CoV -2 virus, Sensitive Detection, SARS-CoV -2, COVID -19-positive patients, S genes, 10 COVID -19-negative subjects, COVID -19 pandemic outbreak, Multiplexed Magnetofluorescent Biop. |
| الوصف: |
Rapid and sensitive detection of SARS-CoV-2 virus genetic material is of paramount importance to mitigate the COVID-19 pandemic outbreak and lower the death toll. Herein, we report the design of a magnetofluorescent bioplatform for the direct and specific detection of the viral RNA of SARS-CoV-2 in the total RNA extracted from nasopharyngeal swabs of COVID-19-positive patients. A higher fluorescence response was achieved using two capture probes tethered to magnetic beads using a biotin/streptavidin linkage, targeting two specific sites in the ORF1a and S genes. Two horseradish peroxidase (HRP)-conjugated reporter sequences, complementary to the loci of the S and N genes, were used to reveal the presence of the viral RNA through the oxidation of o -phenylenediamine to fluorescent 2,3-diaminophenazine. Under optimal conditions, the bioplatform showed high selectivity and sensitivity and was able to detect as low as 0.01 ng of viral RNA (1 × 10 3 copies/μL) with a linear dynamic range varying from 0.01 to 3.0 ng (1 × 10 3 to 9 × 10 7 copies/μL). The bioplatform was also able to discriminate the SARS-CoV-2 RNA from those of other related viruses such as hepatitis C, West Nile, measles, and non-polio viruses. Furthermore, the developed biosensor was validated in 46 clinical samples (36 COVID-19-positive patients and 10 COVID-19-negative subjects, as assessed with the gold standard RT-qPCR method). Both sensitivity and specificity of the developed method reached 100%. Finally, making such a simple and specific method available in the field, at a primary point of care, can better help the detection of SARS-CoV-2 infection in low-resource settings. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Multiplexed_Magnetofluorescent_Bioplatform_for_the_Sensitive_Detection_of_SARS-CoV_2_Viral_RNA_without_Nucleic_Acid_Amplification/15090877Test |
| DOI: |
10.1021/acs.analchem.1c01950.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.analchem.1c01950.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.2C0E9D4C |
| قاعدة البيانات: |
BASE |
