Collagen-producing lung cell atlas identifies multiple subsets with distinct localization and relevance to fibrosis
| العنوان: | Collagen-producing lung cell atlas identifies multiple subsets with distinct localization and relevance to fibrosis |
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| المؤلفون: | Jonas C. Schupp, Lisa A Hazelwood, Dean Sheppard, Ivan O. Rosas, John R. Wilson-Kanamori, J. Wetter, Naftali Kaminski, Michael A. Matthay, Neil C. Henderson, Tatsuya Tsukui, Sergio Poli, Taylor Adams, Kai-Hui Sun, Paul J. Wolters |
| المصدر: | Nature communications, vol 11, iss 1 Tsukui, T, Sun, K-H, Wetter, J B, Wilson-Kanamori, J R, Hazelwood, L A, Henderson, N C, Adams, T S, Schupp, J C, Poli, S D, Rosas, I O, Kaminski, N, Matthay, M A, Wolters, P J & Sheppard, D 2020, ' Collagen-producing lung cell atlas identifies multiple subsets with distinct localization and relevance to fibrosis ', Nature Communications, vol. 11, no. 1, pp. 1920 . https://doi.org/10.1038/s41467-020-15647-5Test Nature Communications, Vol 11, Iss 1, Pp 1-16 (2020) Nature Communications |
| بيانات النشر: | eScholarship, University of California, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Adoptive cell transfer, Pathology, Pulmonary Fibrosis, General Physics and Astronomy, Lung/metabolism, Cell Separation, Inbred C57BL, Idiopathic pulmonary fibrosis, Mice, 0302 clinical medicine, Single-cell analysis, Fibrosis, Pulmonary fibrosis, 2.1 Biological and endogenous factors, Aetiology, lcsh:Science, Lung, Fibroblasts/metabolism, Extracellular Matrix Proteins, Multidisciplinary, High-Throughput Nucleotide Sequencing, respiratory system, Flow Cytometry, medicine.anatomical_structure, Phenotype, 030220 oncology & carcinogenesis, Respiratory, Female, Collagen, Single-Cell Analysis, Collagen/chemistry, Biotechnology, medicine.medical_specialty, Science, Green Fluorescent Proteins, In situ hybridization, Biology, Autoimmune Disease, General Biochemistry, Genetics and Molecular Biology, Article, Extracellular Matrix Proteins/metabolism, 03 medical and health sciences, Pulmonary Fibrosis/metabolism, Respiration Disorders/metabolism, medicine, Animals, Humans, Green Fluorescent Proteins/metabolism, Respiratory tract diseases, General Chemistry, Fibroblasts, medicine.disease, Respiration Disorders, Idiopathic Pulmonary Fibrosis, respiratory tract diseases, Mice, Inbred C57BL, 030104 developmental biology, Idiopathic Pulmonary Fibrosis/pathology, Next-generation sequencing, lcsh:Q, Immunostaining |
| الوصف: | Collagen-producing cells maintain the complex architecture of the lung and drive pathologic scarring in pulmonary fibrosis. Here we perform single-cell RNA-sequencing to identify all collagen-producing cells in normal and fibrotic lungs. We characterize multiple collagen-producing subpopulations with distinct anatomical localizations in different compartments of murine lungs. One subpopulation, characterized by expression of Cthrc1 (collagen triple helix repeat containing 1), emerges in fibrotic lungs and expresses the highest levels of collagens. Single-cell RNA-sequencing of human lungs, including those from idiopathic pulmonary fibrosis and scleroderma patients, demonstrate similar heterogeneity and CTHRC1-expressing fibroblasts present uniquely in fibrotic lungs. Immunostaining and in situ hybridization show that these cells are concentrated within fibroblastic foci. We purify collagen-producing subpopulations and find disease-relevant phenotypes of Cthrc1-expressing fibroblasts in in vitro and adoptive transfer experiments. Our atlas of collagen-producing cells provides a roadmap for studying the roles of these unique populations in homeostasis and pathologic fibrosis. Collagen production by lung cells is critical to maintain organ architecture but can also drive pathological scarring. Here the authors perform single cell RNA sequencing of collagen-producing lung cells identifying a subset of pathologic fibroblasts characterized by Cthrc1 expression which are concentrated within fibroblastic foci in fibrotic lungs and show a pro-fibrotic phenotype. |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df08bc653570e2bcae6b20b7e089763cTest https://escholarship.org/uc/item/5n17z80zTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....df08bc653570e2bcae6b20b7e089763c |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |