دورية أكاديمية
A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection
| العنوان: | A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection |
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| المؤلفون: | Ercan, Huriye (Medical University of Vienna / Immunology Outpatient Clinic), Zellner, Maria (Medical University of Vienna), Miller, Ingrid (University of Veterinary Medicine, Vienna), Geiger, Margarethe (Medical University of Vienna), Yang, Jae-Won (Medical University of Vienna), Gerner, Christopher (University of Vienna), Bileck, Andrea (University of Vienna), Mitulovic, Goran (Medical University of Vienna), Resch, Ulrike (Medical University of Vienna), Hsu, Felicia (Medical University of Vienna) |
| المصدر: | Cells 12(5) (2023) |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Label-Free Quantification, 2-Dimensional Electrophoresis, Biological Variation, Alzheimers-Disease, Apolipoprotein-E, 2-D Dige, Protein, Shotgun, Identification, Purification |
| الوصف: | Proteomics is an indispensable analytical technique to study the dynamic functioning of biological systems via different proteins and their proteoforms. In recent years, bottom-up shotgun has become more popular than gel-based top-down proteomics. The current study examined the qualitative and quantitative performance of these two fundamentally different methodologies by the parallel measurement of six technical and three biological replicates of the human prostate carcinoma cell line DU145 using its two most common standard techniques, label-free shotgun and two-dimensional differential gel electrophoresis (2D-DIGE). The analytical strengths and limitations were explored, finally focusing on the unbiased detection of proteoforms, exemplified by discovering a prostate cancer-related cleavage product of pyruvate kinase M2. Label-free shotgun proteomics quickly yields an annotated proteome but with reduced robustness, as determined by three times higher technical variation compared to 2D-DIGE. At a glance, only 2D-DIGE top-down analysis provided valuable, direct stoichiometric qualitative and quantitative information from proteins to their proteoforms, even with unexpected post-translational modifications, such as proteolytic cleavage and phosphorylation. However, the 2D-DIGE technology required almost 20 times as much time per protein/proteoform characterization with more manual work. Ultimately, this work should expose both techniques' orthogonality with their different contents of data output to elucidate biological questions. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | isPartOf:https://phaidra.vetmeduni.ac.at/o:605Test[Publications / University of Veterinary Medicine Vienna]; https://phaidra.vetmeduni.ac.at/o:1504Test |
| DOI: | 10.3390/cells12050747 |
| الإتاحة: | https://doi.org/10.3390/cells12050747Test https://phaidra.vetmeduni.ac.at/o:1504Test |
| حقوق: | CC BY 4.0 International ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.14C7A978 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/cells12050747 |
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