دورية أكاديمية
Synaptotagmin-11 facilitates assembly of a presynaptic signaling complex in post-Golgi cargo vesicles.
| العنوان: | Synaptotagmin-11 facilitates assembly of a presynaptic signaling complex in post-Golgi cargo vesicles. |
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| المؤلفون: | Trovò, Luca, Kouvaros, Stylianos, Schwenk, Jochen, Fernandez-Fernandez, Diego, Fritzius, Thorsten, Rem, Pascal Dominic, Früh, Simon, Gassmann, Martin, Fakler, Bernd, Bischofberger, Josef, Bettler, Bernhard |
| المصدر: | EMBO Rep ; ISSN:1469-3178 ; Volume:25 ; Issue:6 |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2024 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | Cav2.2, GABA-B, KCTD16, Receptor Signaling Complex Assembly, Transport Vesicle |
| الوصف: | GABAB receptors (GBRs), the G protein-coupled receptors for GABA, regulate synaptic transmission throughout the brain. A main synaptic function of GBRs is the gating of Cav2.2-type Ca2+ channels. However, the cellular compartment where stable GBR/Cav2.2 signaling complexes form remains unknown. In this study, we demonstrate that the vesicular protein synaptotagmin-11 (Syt11) binds to both the auxiliary GBR subunit KCTD16 and Cav2.2 channels. Through these dual interactions, Syt11 recruits GBRs and Cav2.2 channels to post-Golgi vesicles, thus facilitating assembly of GBR/Cav2.2 signaling complexes. In addition, Syt11 stabilizes GBRs and Cav2.2 channels at the neuronal plasma membrane by inhibiting constitutive internalization. Neurons of Syt11 knockout mice exhibit deficits in presynaptic GBRs and Cav2.2 channels, reduced neurotransmitter release, and decreased GBR-mediated presynaptic inhibition, highlighting the critical role of Syt11 in the assembly and stable expression of GBR/Cav2.2 complexes. These findings support that Syt11 acts as a vesicular scaffold protein, aiding in the assembly of signaling complexes from low-abundance components within transport vesicles. This mechanism enables insertion of pre-assembled functional signaling units into the synaptic membrane. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1038/s44319-024-00147-0Test; https://pubmed.ncbi.nlm.nih.gov/38698221Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11169412Test/ |
| DOI: | 10.1038/s44319-024-00147-0 |
| الإتاحة: | https://doi.org/10.1038/s44319-024-00147-0Test https://pubmed.ncbi.nlm.nih.gov/38698221Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11169412Test/ |
| حقوق: | © 2024. The Author(s). |
| رقم الانضمام: | edsbas.A4390B79 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s44319-024-00147-0 |
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