Impaired T-bet-pSTAT1α and perforin-mediated immune responses in the tumoral region of lung adenocarcinoma
| العنوان: | Impaired T-bet-pSTAT1α and perforin-mediated immune responses in the tumoral region of lung adenocarcinoma |
|---|---|
| المؤلفون: | Arndt Hartmann, Susetta Finotto, Raphaela Siegemund, Katerina Andreev, I Denis Iulian Trufa, Joachim Schmidt, Ralf J. Rieker, Horia Sirbu |
| المصدر: | British Journal of Cancer |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, Cancer Research, Lung Neoplasms, medicine.medical_treatment, Programmed Cell Death 1 Receptor, Adenocarcinoma, CD8-Positive T-Lymphocytes, T-bet, NSCLC, Interferon-gamma, Young Adult, Lymphocytes, Tumor-Infiltrating, Immune system, lung tumour, Carcinoma, Non-Small-Cell Lung, medicine, Humans, Protein Isoforms, Cytotoxic T cell, Lymphocyte Count, RNA, Messenger, Lung cancer, IFN-γ, Aged, lung T cells, Aged, 80 and over, Paraffin Embedding, biology, Perforin, Interferon-Stimulated Gene Factor 3, Immunotherapy, Middle Aged, medicine.disease, Neoplasm Proteins, respiratory tract diseases, Cytokine, Oncology, Immunology, Carcinoma, Squamous Cell, biology.protein, Cytokines, Female, Translational Therapeutics, Corrigendum, T-Box Domain Proteins, CD8 |
| الوصف: | Background: In spite of modern therapies for non-small-cell lung cancer (NSCLC), prognosis for many patients is still poor and survival rates are low. Immunotherapy is the possibility to improve the lung immune response surrounding the tumour. However, this approach requires detailed understanding of the local immune-responses of NSCLC patients. Methods: We analysed samples from three different regions within the lungs of NSCLC patients, whereas we distinguished between patients suffering from adenocarcinoma and squamous cell carcinoma. Expression of type 1 T helper (Th1)/type 1 cytotoxic (Tc1) factors was assessed by quantitative real-time PCR, western blot analyses or immunohistochemistry. Cytotoxic cell activity of CD8+ T cells was determined via co-culture with autologous tumour cells and apoptosis assay. Results: We found decreased levels of the transcription factor T-box expressed in T cells (T-bet or Tbx21) and of the downstream activated IFN-γ-dependent pSTAT1α isoform in the lung tumour areas of patients with NSCLC as compared with tumour-free control regions. In these patients, reduced T-bet and pSTAT1α levels were found associated with increased immunosuppressive markers like cytotoxic T lymphocyte-associated protein 4, programmed cell death 1 and with a suppression of the Th1 cell cytokine production and Tc1 cell activity. Conclusions: These findings confirm a central role of T-bet in targeted immunotherapy for patients with NSCLC. |
| تدمد: | 1532-1827 0007-0920 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7b571c57dce4e63f776542a40b915efTest https://doi.org/10.1038/bjc.2015.255Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e7b571c57dce4e63f776542a40b915ef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15321827 00070920 |
|---|