دورية أكاديمية
Change in prostaglandin expression levels and synthesizing activities in dry eye disease
| العنوان: | Change in prostaglandin expression levels and synthesizing activities in dry eye disease |
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| المساهمون: | Jongwoo Shim, Changhun Park, Hyun Soo Lee, Min Soo Park, Hyung Taek Lim, Sunil Chauhan, Reza Dana, Hyon Lee, Hyung Keun Lee, Park, Min Soo, Lee, Hyung Keun |
| المصدر: | T201205088.pdf |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Adult, Aged, 80 and over, Animals, Case-Control Studies, Chromatography, High Pressure Liquid, Cornea/metabolism, Cross-Sectional Studies, Cyclooxygenase 1/genetics, Cyclooxygenase 2/genetics, Dinoprostone/genetics, Dinoprostone/metabolism, Disease Models, Animal, Dry Eye Syndromes/metabolism, Epoprostenol/genetics, Epoprostenol/metabolism, Female, Humans, Immunoenzyme Techniques, Intramolecular Oxidoreductases/genetics, Lacrimal Apparatus/metabolism, Lipocalins/genetics, Male, Meibomian Glands/metabolism, Mice, Inbred C57BL, Middle Aged, Prostaglandin D2/genetics |
| الوصف: | OBJECTIVE: To investigate the expression level of prostaglandins (PGs) and their de novo synthesis in dry eye (DE) disease. DESIGN: Cross-sectional case-control study and in vivo mouse experimental study. PARTICIPANTS: Forty-six eyes from 23 DE patients and 33 eyes from 17 age- and sex-matched controls were studied. Also, DE-induced murine eyes were compared with control eyes. METHODS: Patients completed a symptom questionnaire using a 100-mm visual analog scale (VAS). Nano-liquid chromatography tandem mass spectrometry was used for the quantification of PGE2 and PGD2. A DE disease environmental chamber was used to induce DE in mice. One week after induction, enzyme expressions of cyclooxygenase-1, cyclooxygenase-2 (COX-2), PG E synthase (PGES), and PG D synthase (PGDS) in the lacrimal glands, meibomian glands, and corneas were examined using immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR). MAIN OUTCOME MEASURES: The mean PGE2 and PGD2 levels in the tears of DE patients were measured and compared with symptom severity scores. Immunohistochemistry staining patterns and qRT-PCR data of DE mice were quantified. RESULTS: The mean PGE2 level in the tears of DE patients (2.72 짹3 .42 ng/ml) was significantly higher than that in the control group (0.88 짹 0.83 ng/ml; P = 0.003). However, the mean PGD2 level in the tears of DE patients (0.11 짹 0.22 ng/ml) was significantly lower (0.91 짹 3.28 ng/ml; P = 0.028). The mean PGE2-to-PGD2 ratio correlated strongly with VAS scoring (P = 0.008). In DE mice, COX-2 mRNA was significantly higher in ocular surface tissue and lacrimal glands. Furthermore, PGES mRNA was significantly higher in ocular surface tissue, whereas PGDS mRNA was decreased. Immunohistochemistry staining showed elevated COX-2 expression in the lacrimal glands, meibomian glands, corneas, and conjunctivas. Furthermore, PGES expression was found in periductal infiltrated cells of the lacrimal glands and conjunctival epithelium. Also, PGDS expression was decreased in meibomian ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0161-6420 1549-4713 |
| العلاقة: | OPHTHALMOLOGY; J02432; OAK-2012-02563; https://ir.ymlib.yonsei.ac.kr/handle/22282913/91596Test; T201205088; OPHTHALMOLOGY, Vol.119(11) : 2211-2219, 2012 |
| الإتاحة: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91596Test |
| حقوق: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/krTest/ |
| رقم الانضمام: | edsbas.4E4FC1F8 |
| قاعدة البيانات: | BASE |
| تدمد: | 01616420 15494713 |
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