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Efficient synthesis 1,4-cyclohexanedicarboxaldehyde by an engineered alcohol oxidase

التفاصيل البيبلوغرافية
العنوان: Efficient synthesis 1,4-cyclohexanedicarboxaldehyde by an engineered alcohol oxidase
المؤلفون: Yaqi Cheng, Wei Song, Xiulai Chen, Cong Gao, Jia Liu, Liang Guo, Meng Zhu, Liming Liu, Jing Wu
المصدر: Bioresources and Bioprocessing, Vol 9, Iss 1, Pp 1-14 (2022)
بيانات النشر: SpringerOpen, 2022.
سنة النشر: 2022
المجموعة: LCC:Technology
LCC:Chemical technology
LCC:Biotechnology
مصطلحات موضوعية: 1,4-Cyclohexanedicarboxaldehyde, Alcohol oxidase, Primary alcohol oxidation reaction, Protein engineering, Technology, Chemical technology, TP1-1185, Biotechnology, TP248.13-248.65
الوصف: Abstract In this study, we selected and engineered a flavin adenine dinucleotide (FAD)-dependent alcohol oxidase (AOX) to produce 1,4-cyclohexanedicarboxaldehyde (CHDA), an initial raw material for spiral compounds, from 1,4-cyclohexanedimethanol (CHDM). First, the structure of alcohol oxidase from Arthrobacter cholorphenolicus (AcCO) was analyzed, and the mechanism of AcCO-catalyzed primary alcohol oxidation was elucidated, demonstrating that the energy barrier of the hydride (H−) transfer (13.4 kcal·mol−1 and 20.4 kcal·mol−1) decreases the catalytic efficiency of the primary alcohol oxidation reaction. Therefore, we designed a protein engineering strategy to adjust the catalytically active conformation to shorten the distance of hydride (H−) transfer and further decreased the core energy barrier. Following this strategy, variant W4 (S101A/H351V/N378S/Q329N) was obtained with 112.5-fold increased catalytic efficiency to produce CHDA compared to that of the wild-type strain. The 3 L scale preparation of CHDA reached a titer up to 29.6 g·L−1 with a 42.2% yield by an Escherichia coli whole-cell catalyst, which demonstrates the potential of this system for industrial application. Graphical Abstract
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2197-4365
العلاقة: https://doaj.org/toc/2197-4365Test
DOI: 10.1186/s40643-022-00570-y
الوصول الحر: https://doaj.org/article/a7771bd53b0c48b891a4988a3714761dTest
رقم الانضمام: edsdoj.7771bd53b0c48b891a4988a3714761d
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:21974365
DOI:10.1186/s40643-022-00570-y