التفاصيل البيبلوغرافية
| العنوان: |
Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome |
| المؤلفون: |
Yatskevich, Stanislau, Muir, Kyle W., Bellini, Dom, Zhang, Ziguo, Yang, Jing, Tischer, Thomas, Predin, Masa, Dendooven, Tom, McLaughlin, Stephen H., Barford, David |
| المصدر: |
Science ; volume 376, issue 6595, page 844-852 ; ISSN 0036-8075 1095-9203 |
| بيانات النشر: |
American Association for the Advancement of Science (AAAS) |
| سنة النشر: |
2022 |
| الوصف: |
Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A Nuc ) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo–electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-A Nuc reconstituted onto α-satellite DNA. CCAN forms edge-on contacts with CENP-A Nuc , and a linker DNA segment of the α-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1126/science.abn3810 |
| الإتاحة: |
https://doi.org/10.1126/science.abn3810Test |
| رقم الانضمام: |
edsbas.BCC4B360 |
| قاعدة البيانات: |
BASE |
