دورية أكاديمية
Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq
| العنوان: | Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq |
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| المؤلفون: | Restivo, Gaetana, Tastanova, Aizhan, Balázs, Zsolt, Panebianco, Federica, Diepenbruck, Maren, Ercan, Caner, Preca, Bodgan-T, Hafner, Jürg, Weber, Walter P, Kurzeder, Christian, Vetter, Marcus, Soysal, Simone Münst, Beisel, Christian, Bentires-Alj, Mohamed, Piscuoglio, Salvatore, Krauthammer, Michael, Levesque, Mitchell P |
| المصدر: | Restivo, Gaetana; Tastanova, Aizhan; Balázs, Zsolt; Panebianco, Federica; Diepenbruck, Maren; Ercan, Caner; Preca, Bodgan-T; Hafner, Jürg; Weber, Walter P; Kurzeder, Christian; Vetter, Marcus; Soysal, Simone Münst; Beisel, Christian; Bentires-Alj, Mohamed; Piscuoglio, Salvatore; Krauthammer, Michael; Levesque, Mitchell P (2022). Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq. Communications Biology, 5:1144. |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2022 |
| المجموعة: | University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| مصطلحات موضوعية: | Dermatology Clinic, 610 Medicine & health, General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, Medicine (miscellaneous) |
| الوصف: | Biobanking of surplus human healthy and disease-derived tissues is essential for diagnostics and translational research. An enormous amount of formalin-fixed and paraffin-embedded (FFPE), Tissue-Tek OCT embedded or snap-frozen tissues are preserved in many biobanks worldwide and have been the basis of translational studies. However, their usage is limited to assays that do not require viable cells. The access to intact and viable human material is a prerequisite for translational validation of basic research, for novel therapeutic target discovery, and functional testing. Here we show that surplus tissues from multiple solid human cancers directly slow-frozen after resection can subsequently be used for different types of methods including the establishment of 2D, 3D, and ex vivo cultures as well as single-cell RNA sequencing with similar results when compared to freshly analyzed material. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2399-3642 |
| العلاقة: | https://www.zora.uzh.ch/id/eprint/224947/1/ZORA_s42003_022_04025_0.pdfTest; info:pmid/36307545; urn:issn:2399-3642 |
| DOI: | 10.5167/uzh-224947 |
| DOI: | 10.1038/s42003-022-04025-0 |
| الإتاحة: | https://doi.org/10.5167/uzh-22494710.1038/s42003-022-04025-0Test https://www.zora.uzh.ch/id/eprint/224947Test/ https://www.zora.uzh.ch/id/eprint/224947/1/ZORA_s42003_022_04025_0.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess ; Creative Commons: Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.7C3669A5 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 23993642 |
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| DOI: | 10.5167/uzh-224947 |