دورية أكاديمية
OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus
| العنوان: | OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus |
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| المؤلفون: | Castaño-Rodríguez, N, Popple, GL, Porras-Hurtado, GL, Cardona-Deazza, JL, Montoya-Martinez, JJ, Cadavid-Velez, AJ, Toro-Hidalgo, HW, Cobo-Alvarado, AR, Del Socorro Hincapié-Rincón, O, Riordan, SM, Kaakoush, NO |
| المصدر: | urn:ISSN:2050-7771 ; Biomarker Research, 6, 1, 35 |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2018 |
| المجموعة: | UNSW Sydney (The University of New South Wales): UNSWorks |
| مصطلحات موضوعية: | Clinical Research, Rare Diseases, Digestive Diseases, Cancer, Adenocarcinoma, CpG methylation, Dysplasia, OPCML |
| الوصف: | OPCML hypermethylation is considered a promising cancer biomarker. We examined methylation levels in the first exon of OPCML in two patient cohorts within the esophageal adenocarcinoma and gastric adenocarcinoma cascades and in a range of cell-lines using a custom PyroMark CpG assay. Methylation levels were significantly higher in esophageal tissue with histologically confirmed glandular mucosa as compared to tissue from normal esophagi or gastro-esophageal reflux disease. Higher levels of OPCML methylation were absent in the adjacent normal esophageal tissue of patients with glandular mucosa. Higher levels of methylation were confirmed in cell-lines derived from patients with adenocarcinoma, but also detected in two cell-lines with signs of dysplasia. We validated our assay by showing no differences in methylation levels in DNA extracted from blood of patients within the gastric adenocarcinoma cascade. OPCML hypermethylation is present in a subset of patients with metaplastic changes in their esophagus. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://purl.org/au-research/grants/nhmrc/APP1111461Test; http://purl.org/au-research/grants/nhmrc/APP1129488Test; http://hdl.handle.net/1959.4/unsworks_55137Test; https://unsworks.unsw.edu.au/bitstreams/67b69503-ef7e-4aad-8328-daedff9591ec/downloadTest; https://doi.org/10.1186/s40364-018-0150-yTest |
| DOI: | 10.1186/s40364-018-0150-y |
| الإتاحة: | https://doi.org/10.1186/s40364-018-0150-yTest http://hdl.handle.net/1959.4/unsworks_55137Test https://unsworks.unsw.edu.au/bitstreams/67b69503-ef7e-4aad-8328-daedff9591ec/downloadTest |
| حقوق: | open access ; https://purl.org/coar/access_right/c_abf2Test ; CC BY ; https://creativecommons.org/licenses/by/4.0Test/ ; free_to_read |
| رقم الانضمام: | edsbas.8D3DBC09 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s40364-018-0150-y |
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