يعرض 1 - 7 نتائج من 7 نتيجة بحث عن '"Polymerase Chain Reaction Product"', وقت الاستعلام: 1.12s تنقيح النتائج
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    دورية أكاديمية
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    دورية أكاديمية

    المؤلفون: SAKSONO, BUDI, SUKMARINI, LINDA

    المصدر: HAYATI Journal of Biosciences; Vol. 17 No. 4 (2010): December 2010; 189 ; 2086-4094 ; 1978-3019

    الوقت: 227, 228, 233

    وصف الملف: application/pdf

    العلاقة: http://journal.ipb.ac.id/index.php/hayati/article/view/%20%3Cp%20class%3D%22MsoNormal%22%20style%3D%22text-align%3Ajustify%3Btext-indent%3A14.15pt%3B%22%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3EA%20xylanase%20gene,%20%3Cem%3ExynA%3C%2Fem%3E,%20has%20been%20cloned%20from%20thermophilic%20strain%20%3Cem%3EGeobacillus%20stearothermophilus%3C%2Fem%3E,%20which%20was%20isolated%20from%20marine%20Tanjung%20Api,%20Indonesia.%20The%20polymerase%20chain%20reaction%20product%20of%201266%20bp%20of%20%3Cem%3ExynA%3C%2Fem%3E%20gene%20consisted%20of%201221%20bp%20open%20reading%20frame%20and%20encoded%20407%20amino%20acids%20including%2030%20residues%20of%20signal%20peptide.%20The%20sequence%20exhibited%20highest%20identity%20of%2098.7%25%20in%20the%20level%20of%20amino%20acid,%20with%20an%20extracellular%20endo-1,4-%3Cem%3E%CE%B2%3C%2Fem%3E-xylanase%20from%20%3Cem%3EG.%20stearothermophilus%3C%2Fem%3E%20T-6%20%28E-GSX%20T-6%29%20of%20the%20glycoside%20hydrolase%20family%2010%20%28GH10%29.%20A%20comparative%20study%20between%20the%20local%20strain%20%3Cem%3EG.%20stearothermophilus%3C%2Fem%3E%20%28GSX%20L%29%20and%20E-GSX%20T-6%20on%20homology%20of%20amino%20acid%20sequence%20indicated%20five%20differents%20amino%20acids%20in%20the%20gene.%20They%20were%20Threonine%2FAlanine%20%28T%2FA%29,%20Asparagine%2FAspartate%20%28N%2FD%29,%20Lysine%2FAsparagine%20%28K%2FN%29,%20Isoleucine%2FMethionine%20%28I%2FM%29,%20Serine%2FThreonine%20%28S%2FT%29%20at%20the%20position%20220,%20227,%20228,%20233,%20and%20245,%20respectively.%20Protein%20structural%20analysis%20of%20those%20differences%20suggested%20that%20those%20amino%20acids%20may%20play%20role%20in%20biochemical%20properties%20such%20as%20enzyme%20stability,%20in%20particular%20its%20thermostability.%3C%2Fspan%3E%3C%2Fstrong%3E%3C%2Fp%3E/1228Test

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    كتاب

    المساهمون: Carmichael, Gordon G.

    المصدر: Clayton , C E , Estévez , A M , Hartmann , C , Alibu , V P , Field , M & Horn , D 2005 , Down-regulating gene expression by RNA interference in Trypanosoma brucei . in G G Carmichael (ed.) , RNA silencing : Methods and protocols . vol. 309 , Methods in Molecular Biology , Springer , pp. 39-60 . https://doi.org/10.1385/1592599354Test

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