Water Sorption and Structural Properties of Human Airway Mucus in Health and Muco-Obstructive Diseases.
| العنوان: | Water Sorption and Structural Properties of Human Airway Mucus in Health and Muco-Obstructive Diseases. |
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| المؤلفون: | Kelly, Susyn J, Genevskiy, Vladislav, Björklund, Sebastian, Gonzalez-Martinez, Juan F, Poeschke, Lara, Schröder, Maik, Nilius, Georg, Tatkov, Stanislav, Kocherbitov, Vitaly |
| المصدر: | Biomacromolecules. 25(3):1578-1591 |
| الوصف: | Muco-obstructive diseases change airway mucus properties, impairing mucociliary transport and increasing the likelihood of infections. To investigate the sorption properties and nanostructures of mucus in health and disease, we investigated mucus samples from patients and cell cultures (cc) from healthy, chronic obstructive pulmonary disease (COPD), and cystic fibrosis (CF) airways. Atomic force microscopy (AFM) revealed mucin monomers with typical barbell structures, where the globule to spacer volume ratio was the highest for CF mucin. Accordingly, synchrotron small-angle X-ray scattering (SAXS) revealed more pronounced scattering from CF mucin globules and suggested shorter carbohydrate side chains in CF mucin and longer side chains in COPD mucin. Quartz crystal microbalance with dissipation (QCM-D) analysis presented water sorption isotherms of the three types of human airway mucus, where, at high relative humidity, COPD mucus had the highest water content compared to cc-CF and healthy airway mucus (HAM). The higher hydration of the COPD mucus is consistent with the observation of longer side chains of the COPD mucins. At low humidity, no dehydration-induced glass transition was observed in healthy and diseased mucus, suggesting mucus remained in a rubbery state. However, in dialyzed cc-HAM, a sorption-desorption hysteresis (typically observed in the glassy state) appeared, suggesting that small molecules present in mucus suppress the glass transition. |
| وصف الملف: | electronic |
| الوصول الحر: | https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-66269Test https://doi.org/10.1021/acs.biomac.3c01170Test https://mau.diva-portal.org/smash/get/diva2:1843158/FULLTEXT01.pdfTest |
| قاعدة البيانات: | SwePub |
| تدمد: | 15257797 15264602 |
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| DOI: | 10.1021/acs.biomac.3c01170 |