التفاصيل البيبلوغرافية
| العنوان: |
Comparison of the Efficiency of Various Promoters for the Production of Secreted β-Mannanase by Bacillus subtilis Cells of the Methylotrophic Yeast Ogataea haglerorum. |
| المؤلفون: |
Podpletnev, D. A.1 (AUTHOR), Lapteva, A. R.1 (AUTHOR), Sineoky, S. P.1 (AUTHOR), Tarutina, M. G.1,2 (AUTHOR) m_tarutina@mail.ru |
| المصدر: |
Applied Biochemistry & Microbiology. Mar2024, Vol. 60 Issue 1, p38-45. 8p. |
| مصطلحات موضوعية: |
*PICHIA pastoris, *BACILLUS subtilis, *GENETIC regulation, *YEAST, *GENE expression, *REPORTER genes, *RECOMBINANT proteins |
| مستخلص: |
In this article, strong promoters of thermotolerant methylotrophic yeast Ogataea haglerorum have been characterized. Promoters play a key role in the regulation of gene expression; therefore, they are the important element of expression vectors. Strong and strictly regulated promoters are a powerful tool for creating highly productive strains–producers of recombinant proteins. To expand the potential of the O. haglerorum expression system natural methanol-induced promoters of the OhMOX and OhFMD genes and the constitutive promoter of the OhGAP gene were studied in comparison with the promoter of the MOX gene from O. polymorpha yeast. A gene encoding recombinant β-mannanase was used as a reporter gene. It has been shown that in O. haglerorum yeast cells, the expression level (strength) of the pOhMOX promoter is about 1.4–1.9 times higher relative to the pOpMOX promoter from O. polymorpha yeast. The data on the strength of promoters from yeast O. haglerorum can be useful in designing producers of recombinant proteins and optimizing metabolic pathways in methylotrophic yeast O. haglerorum. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
