دورية أكاديمية
An improved pathway for autonomous bioluminescence imaging in eukaryotes
| العنوان: | An improved pathway for autonomous bioluminescence imaging in eukaryotes |
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| المؤلفون: | Shakhova, Ekaterina S., Karataeva, Tatiana A., Markina, Nadezhda M., Mitiouchkina, Tatiana, Palkina, Kseniia A., Perfilov, Maxim M., Wood, Monika G., Hoang, Trish T., Hall, Mary P., Fakhranurova, Liliia I., Alekberova, Anna E., Malyshevskaia, Alena K., Gorbachev, Dmitry A., Bugaeva, Evgenia N., Pletneva, Ludmila K., Babenko, Vladislav V., Boldyreva, Daria I., Gorokhovatsky, Andrey Y., Balakireva, Anastasia V., Gao, Feng, Choob, Vladimir V., Encell, Lance P., Wood, Keith V., Yampolsky, Ilia V., Sarkisyan, Karen S., Mishin, Alexander S. |
| المساهمون: | RCUK | Medical Research Council, Planta, Promega, Light Bio, Russian Science Foundation |
| المصدر: | Nature Methods ; ISSN 1548-7091 1548-7105 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Cell Biology, Molecular Biology, Biochemistry, Biotechnology |
| الوصف: | The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41592-023-02152-y |
| الإتاحة: | https://doi.org/10.1038/s41592-023-02152-yTest https://www.nature.com/articles/s41592-023-02152-y.pdfTest https://www.nature.com/articles/s41592-023-02152-yTest |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.373EEB0F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41592-023-02152-y |
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