دورية أكاديمية
Tomato (Solanum lycopersicum L.) accumulation and allergenicity in response to nickel stress
| العنوان: | Tomato (Solanum lycopersicum L.) accumulation and allergenicity in response to nickel stress |
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| المؤلفون: | Roccotiello E., Nicosia E., Pierdona L., Marescotti P., Ciardiello M. A., Giangrieco I., Mari A., Zennaro D., Dozza D., Brancucci M., Mariotti M. |
| المساهمون: | Roccotiello, E., Nicosia, E., Pierdona, L., Marescotti, P., Ciardiello, M. A., Giangrieco, I., Mari, A., Zennaro, D., Dozza, D., Brancucci, M., Mariotti, M. |
| بيانات النشر: | Nature Research DEU Berlino |
| سنة النشر: | 2022 |
| المجموعة: | Università degli Studi di Genova: CINECA IRIS |
| مصطلحات موضوعية: | Fruit, Profilin, Allergen, Lycopersicon esculentum, Nickel |
| الوصف: | Vegetables represent a major source of Ni exposure. Environmental contamination and cultural practices can increase Ni amount in tomato posing significant risk for human health. This work assesses the tomato (Solanum lycopersicum L.) response to Ni on the agronomic yield of fruits and the related production of allergens. Two cultivars were grown in pots amended with Ni 0, 30, 60, 120, and 300mgkg−1, respectively. XRF and ICP-MS analyses highlighted the direct increase of fruit Ni content compared to soil Ni, maintaining a stable biomass. Leaf water content increased at Ni 300mgkg−1. Total protein content and individual allergenic components were investigated using biochemical (RP-HPLC and N-terminal amino acid sequencing) and immunological (inhibition tests of IgE binding by SPHIAa assay on the FABER testing system) methodologies. Ni affected the fruit tissue concentration of pathogenesis-related proteins and relevant allergens (LTP, profilin, Bet v 1-like protein and TLP). This study elucidates for the first time that tomato reacts to exogenous Ni, uptaking the metal while changing its allergenic profiles, with potential double increasing of exposure risks for consumers. This evidence highlighted the importance of adequate choice of low-Ni tomato cultivars and practices to reduce Ni uptake by potentially contaminated matrices. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/35361841; info:eu-repo/semantics/altIdentifier/wos/WOS:000776953600049; volume:12; firstpage:1; lastpage:15; numberofpages:15; journal:SCIENTIFIC REPORTS; http://hdl.handle.net/11567/1080116Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85127427363 |
| DOI: | 10.1038/s41598-022-09107-x |
| الإتاحة: | https://doi.org/10.1038/s41598-022-09107-xTest http://hdl.handle.net/11567/1080116Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.35A2CD03 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-022-09107-x |
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