دورية أكاديمية
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) of endonuclease digests of RNA
| العنوان: | Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) of endonuclease digests of RNA |
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| المؤلفون: | Hahner, Stephanie, Lüdemann, Hans-Christian, Kirpekar, Finn, Nordhoff, Eckhard, Roepstorff, P, Galla, H J, Hillenkamp, F |
| المصدر: | Hahner , S , Lüdemann , H-C , Kirpekar , F , Nordhoff , E , Roepstorff , P , Galla , H J & Hillenkamp , F 1997 , ' Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) of endonuclease digests of RNA ' , Nucleic Acids Research , vol. 25 , no. 10 , pp. 1957-64 . https://doi.org/10.1093/nar/25.10.1957Test |
| سنة النشر: | 1997 |
| المجموعة: | University of Southern Denmark: Research Output / Syddansk Universitet |
| مصطلحات موضوعية: | Animals, Aspergillus oryzae/enzymology, Base Sequence, Cattle, Chickens, Liver/enzymology, Molecular Sequence Data, Oligoribonucleotides/chemical synthesis, Pancreas/enzymology, Physarum/enzymology, Plants, RNA/biosynthesis, Ribonuclease T1/metabolism, Ribonucleases/metabolism, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, Substrate Specificity, Transcription, Genetic, Ustilago/enzymology |
| الوصف: | The determination of RNA sequences using base- specific enzymatic cleavages is a well established method. Different synthetic RNA molecules were analyzed for uniformity of degradation by RNase T1, U2, A and PhyM under reaction conditions compatible with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS), to identify the positions of G, A and pyrimidine residues. In order to get a complete set of fragments derived from cleavage at every phosphodiester bond, the samples were also subjected to a limited alkaline hydrolysis. Additionally, the 5'-terminus fragments of a 49mer RNA transcript were isolated by way of 5'-biotinylation and streptavidin-coated magnetic beads (Dynal), followed by a RNase U2digestion. MALDI-MS of the generated fragments is presented as an efficient technique for a direct read out of the nucleotide sequence. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://portal.findresearcher.sdu.dk/da/publications/bc74f9b2-2013-42e5-ac76-093afe32eed9Test |
| DOI: | 10.1093/nar/25.10.1957 |
| الإتاحة: | https://doi.org/10.1093/nar/25.10.1957Test https://portal.findresearcher.sdu.dk/da/publications/bc74f9b2-2013-42e5-ac76-093afe32eed9Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.5CAA5778 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/25.10.1957 |
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