دورية أكاديمية
Physical proximity of sister chromatids promotes top2-dependent intertwining
| العنوان: | Physical proximity of sister chromatids promotes top2-dependent intertwining |
|---|---|
| المؤلفون: | Sen, N, Leonard, J, Torres, R, Garcia-Luis, J, Palou-Marin, G, Aragon Alcaide, L |
| المساهمون: | Wellcome Trust |
| المصدر: | 147 ; 134 |
| بيانات النشر: | Elsevier (Cell Press) |
| سنة النشر: | 2016 |
| المجموعة: | Imperial College London: Spiral |
| مصطلحات موضوعية: | Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Cell Biology, MITOTIC CHROMOSOME CONDENSATION, TOPOISOMERASE-II, SACCHAROMYCES-CEREVISIAE, DNA TOPOISOMERASES, IN-VITRO, COHESION, DECATENATION, TOPOLOGY, PROTEIN, MITOSIS, DNA replication, SCI, Top2 (Topoisomerase 2), catenation, chromosome segregation, cohesin, condensin, Adenosine Triphosphatases, Anaphase, Chromatids, Chromosomes, Fungal, Type II, DNA, DNA-Binding Proteins, G2 Phase Cell Cycle Checkpoints |
| الوصف: | Sister chromatid intertwines (SCIs), or catenanes, are topological links between replicated chromatids that interfere with chromosome segregation. The forma- tion of SCIs is thought to be a consequence of fork swiveling during DNA replication, and their removal is thought to occur because of the intrinsic feature of type II topoisomerases (Top2) to simplify DNA topology. Here, we report that SCIs are also formed independently of DNA replication during G 2 /M by Top2-dependent concatenation of cohesed chroma- tids due to their physical proximity. We demonstrate that, in contrast to G 2 /M, Top2 removes SCIs from co- hesedchromatidsattheanaphaseonset.Importantly, SCI removal in anaphase requires condensin and co- incides with the hyperactivation of condensin DNA supercoiling activity. This is consistent with the long- standing proposal that condensin provides a bias in Top2functiontowarddecatenation.Acomprehensive model for the formation and resolution of toxic SCI entanglements on eukaryotic genomes is proposed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1097-4164 |
| العلاقة: | Molecular Cell; http://hdl.handle.net/10044/1/40855Test; 100955/Z/13/Z |
| DOI: | 10.1016/j.molcel.2016.09.007 |
| الإتاحة: | https://doi.org/10.1016/j.molcel.2016.09.007Test http://hdl.handle.net/10044/1/40855Test |
| رقم الانضمام: | edsbas.EF1EB1C4 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 10974164 |
|---|---|
| DOI: | 10.1016/j.molcel.2016.09.007 |