دورية أكاديمية
Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study
| العنوان: | Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study |
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| المؤلفون: | Dutysheva, E. A., Mikhaylova, E. R., Trestsova, M. A., Andreev, A. I., Apushkin, D. Y., Utepova, I. A., Serebrennikova, P. O., Akhremenko, E. A., Aksenov, N. D., Bon’, E. I., Zimatkin, S. M., Chupakhin, O. N., Margulis, B. A., Guzhova, I. V., Lazarev, V. F. |
| المصدر: | Pharmaceutics |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Ural Federal University (URFU): ELAR / Уральский федеральный университет: электронный архив УрФУ |
| مصطلحات موضوعية: | 3-(5-PHENYL-1H-PYRROL-2-YL)QUINOXALIN-2(1H)-ONE, GAPDH, HEAT SHOCK PROTEIN, HSP70, HYDROCORTISONE 21-HEMISUCCINATE, REHABILITATION THERAPY, SECONDARY DAMAGE, SMALL MOLECULES, TRAUMATIC BRAIN INJURY, CHAPERONE, GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE, HEAT SHOCK PROTEIN 70, HUNTINGTIN, HYDROCORTISONE, HYDROCORTISONE SODIUM SUCCINATE, PENICILLIN DERIVATIVE, PYRROLYLAZINE DERIVATIVE, STREPTOMYCIN, SUPEROXIDE DISMUTASE, TILETAMINE PLUS ZOLAZEPAM, UNCLASSIFIED DRUG, ZOLETIL 100, ALZHEIMER DISEASE, ANIMAL CELL, ANIMAL EXPERIMENT, ANIMAL MODEL, APOPTOSIS, ARTICLE, BRAIN CORTEX, CELL AGGREGATION |
| الوصف: | The recovery period after traumatic brain injury (TBI) is often complicated by secondary damage that may last for days or even months after trauma. Two proteins, Hsp70 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), were recently described as modulating post-traumatic processes, and in this study, we test them as targets for combination therapy using an inhibitor of GAPDH aggregation (derivative of hydrocortisone RX624) and an inducer of Hsp70 synthesis (the pyrrolylazine derivative PQ-29). The protective effect of the combination on C6 rat glioblastoma cells treated with the cerebrospinal fluid of traumatized animals resulted in an increase in the cell index and in a reduced level of apoptosis. Using a rat weight drop model of TBI, we found that the combined use of both drugs prevented memory impairment and motor deficits, as well as a reduction of neurons and accumulation of GAPDH aggregates in brain tissue. In conclusion, we developed and tested a new approach to the treatment of TBI based on influencing distinct molecular mechanisms in brain cells. © 2022 by the authors. ; Ministry of Education and Science of the Russian Federation, Minobrnauka: 075-15-2021-683; Russian Science Foundation, RSF: 18-74-10087, 22-13-00298 ; This research was funded by the Russian Science Foundation, research projects #18-74-10087 and #22-13-00298. ; Rat glioblastoma C6 cells were obtained from the shared research facility “Vertebrate cell culture collection” supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement No. 075-15-2021-683). Cells were cultured in DMEM/F12 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), 100 units/mL penicillin, and 0.1 mg/mL streptomycin (BioloT, St. Petersburg, Russia) at 37 °C and 5% CO. 2 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4923 |
| العلاقة: | Dutysheva, EA, Mikhaylova, ER, Trestsova, MA, Andreev, AI, Apushkin, DY, Utepova, IA, Serebrennikova, PO, Akhremenko, EA, Aksenov, ND, Bon’, EI, Zimatkin, SM, Chupakhin, ON, Margulis, BA, Guzhova, IV & Lazarev, VF 2023, 'Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study', Pharmaceutics, Том. 15, № 1, 7. https://doi.org/10.3390/pharmaceutics15010007Test; Dutysheva, E. A., Mikhaylova, E. R., Trestsova, M. A., Andreev, A. I., Apushkin, D. Y., Utepova, I. A., Serebrennikova, P. O., Akhremenko, E. A., Aksenov, N. D., Bon’, E. I., Zimatkin, S. M., Chupakhin, O. N., Margulis, B. A., Guzhova, I. V., & Lazarev, V. F. (2023). Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study. Pharmaceutics, 15(1), [7]. https://doi.org/10.3390/pharmaceutics15010007Test; Final; All Open Access, Gold, Green; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85146778461&doi=10.3390%2fpharmaceutics15010007&partnerID=40&md5=9dbb477c2158ebf193dadffce65cf6bcTest; https://www.mdpi.com/1999-4923/15/1/7/pdf?version=1672128532Test; http://elar.urfu.ru/handle/10995/131014Test; 85146778461; 000916065300001 |
| DOI: | 10.3390/pharmaceutics15010007 |
| الإتاحة: | https://doi.org/10.3390/pharmaceutics15010007Test http://elar.urfu.ru/handle/10995/131014Test https://www.mdpi.com/1999-4923/15/1/7/pdf?version=1672128532Test |
| حقوق: | info:eu-repo/semantics/openAccess ; cc-by ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.B0CEA1B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19994923 |
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| DOI: | 10.3390/pharmaceutics15010007 |