Assessment of the Link of ABCB1 and NR3C1 gene polymorphisms with the prednisolone resistance in pediatric nephrotic syndrome patients of Bangladesh: A genotype and haplotype approach
| العنوان: | Assessment of the Link of ABCB1 and NR3C1 gene polymorphisms with the prednisolone resistance in pediatric nephrotic syndrome patients of Bangladesh: A genotype and haplotype approach |
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| المؤلفون: | Most. Nazma Parvin, Mohammad Safiqul Islam, Mohammed Hanif, Md. Saiful Islam, Md. Abdul Aziz, Mir Md. Abdullah Al-Mamun, Sikder Nahidul Islam Rabbi |
| المصدر: | Journal of Advanced Research Journal of Advanced Research, Vol 33, Iss, Pp 141-151 (2021) |
| بيانات النشر: | Elsevier BV, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Medicine (General), CYP3A5, Science (General), Nephrotic Syndrome, Drug Resistance, PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism, NR3C1, MDR1, multidrug resistance gene 1, Gastroenterology, Q1-390, 0302 clinical medicine, Polymorphism (computer science), Genotype, GR, Glucocorticoid receptor, PRNS, Prednisolone resistance nephrotic syndrome, Child, NR3C1, nuclear receptor subfamily 3, group C, member 1, SSNS, Steroid-sensitive nephrotic syndrome, Bangladesh, Multidisciplinary, HWE, Hardy-Weinberg equilibrium, ABCB1, LD, Linkage disequilibrium, 030220 oncology & carcinogenesis, Prednisolone, Medicine, MesPGN, mesangioproliferative glomerulonephritis, PR, Prednisolone resistance, NS, Nephrotic syndrome, PSG, Prednisolone sensitive group, medicine.drug, medicine.medical_specialty, ATP Binding Cassette Transporter, Subfamily B, PRG, Prednisolone resistance group, 03 medical and health sciences, R5-920, GC, Glucocorticoids, Receptors, Glucocorticoid, Internal medicine, medicine, Humans, ComputingMethodologies_COMPUTERGRAPHICS, P-gp, Permeability glycoprotein, Polymorphism, Genetic, business.industry, Haplotype, SRNS, steroid-resistance nephrotic syndrome, medicine.disease, OR, odds ratio, 95%CI, 95% confidence intervals, 030104 developmental biology, Haplotypes, Pharmacogenetics, Prednisolone resistance, Gene polymorphism, business, Nephrotic syndrome |
| الوصف: | Graphical abstract Introduction Nephrotic syndrome is a common pediatric kidney disease. Investigations on several genetic polymorphisms revealed an inconsistent influence on the resistance of patients to steroids. Objectives This study aimed to identify the association of ABCB1 (1236C > T, 2677G > T, 3435C > T), NR3C1 (rs10482634, rs6877893), and CYP3A5 (CYP3A5*3) gene polymorphism as well as sociodemographic and clinicopathological parameters with the risk of developing prednisolone resistance in pediatric patients with nephrotic syndrome. Methods A case-control analysis was performed on 180 nephrotic syndrome patients. Among them, 30 patients were classified as prednisolone resistant group, and 150 were classified as prednisolone sensitive group. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results No significant association of 1236C > T polymorphism with the risk of prednisolone resistance (p > 0.05) was found. The GT heterozygous of 2677G > T was found to be significantly associated with the development of prednisolone resistance (OR = 3.9, p = 0.034). In the case of 3435C > T, a statistically significant association was observed in TC heterozygous and TT mutant homozygous genotypes (OR = 0.38, p = 0.047; OR = 3.06, p = 0.038, respectively) with prednisolone resistance. For rs10482634 polymorphism, the AG heterozygous and AG+GG genotypes were significantly linked with prednisolone resistance (OR = 2.40, p = 0.033; OR = 2.36, p = 0.034, respectively). We found no association with the risk of prednisolone resistance with rs6877893 and CYP3A5*3 polymorphism (p > 0.05). CTC and TGT haplotypes of ABCB1 and GA haplotype of NR3C1 were also associated with the increased risk of pediatric prednisolone resistance (OR = 4.47, p = 0.0003; OR = 2.71, p = 0.03; and OR = 4.22, p = 0.022, consecutively). We also observed the correlation of different sociodemographic and clinicopathological factors with prednisolone resistance in pediatric nephrotic syndrome. Conclusion Our findings showed a significant association of ABCB1 and NR3C1 gene polymorphisms with prednisolone resistant pediatric nephrotic syndrome. |
| تدمد: | 2090-1232 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56cd9b90a915500a6b94ae161c9617daTest https://doi.org/10.1016/j.jare.2021.02.001Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....56cd9b90a915500a6b94ae161c9617da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20901232 |
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