دورية أكاديمية
Optogenetic termination of atrial tachyarrhythmias by brief pulsed light stimulation
| العنوان: | Optogenetic termination of atrial tachyarrhythmias by brief pulsed light stimulation |
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| المؤلفون: | Nakao, M., Watanabe, M., Miquerol, L., Natsui, H., Koizumi, T., Kadosaka, T., Koya, T., Hagiwara, H., Kamada, R., Temma, T., Vries, A.A.F. de, Anzai, T. |
| المصدر: | Journal of Molecular and Cellular Cardiology |
| سنة النشر: | 2023 |
| المجموعة: | Leiden Repository (Leiden University) |
| مصطلحات موضوعية: | Atrial tachyarrhythmia, Optogenetic termination, Channelrhodopsin-2, Action potential |
| الوصف: | Aims The most efficient way to acutely restore sinus rhythm from atrial fibrillation (AF) is electrical cardioversion , which is painful without adequate sedation. Recent studies in various experimental models have indicated that optogenetic termination of AF using light-gated ion channels may provide a myocardium-specific and potentially painless alternative future therapy . However, its underlying mechanism(s) remain(s) incompletely understood. As brief pulsed light stimulation , even without global illumination, can achieve optogenetic AF termination, besides direct conduction block also modulation of action potential (AP) properties may be involved in the termination mechanism. We studied the relationship between optogenetic AP duration (APD) and effective refractory period (ERP) prolongation by brief pulsed light stimulation and termination of atrial tachyarrhythmia (AT). Methods and results Hearts from transgenic mice expressing the H134R variant of channelrhodopsin-2 in atrial myocytes were explanted and perfused retrogradely. AT induced by electrical stimulation was terminated by brief pulsed blue light stimulation (470 nm, 10 ms, 16 mW/mm 2 ) with 68% efficacy. The termination rate was dependent on pulse duration and light intensity. Optogenetically imposed APD and ERP changes were systematically examined and optically monitored. Brief pulsed light stimulation (10 ms, 6 mW/mm 2 ) consistently prolonged APD and ERP when light was applied at different phases of the cardiac action potential . Optical tracing showed light-induced APD prolongation during the termination of AT. Conclusion Our results directly demonstrate that cationic channelrhodopsin activation by brief pulsed light stimulation prolongs the atrial refractory period suggesting that this is one of the key mechanisms of optogenetic termination of AT. ; Cardiology |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-0-02-228282-0 0-02-228282-3 |
| العلاقة: | https://www.sciencedirect.com/science/article/pii/S0022282823000512?via%3DihubTest; lumc-id: 185382049; https://hdl.handle.net/1887/3728753Test |
| DOI: | 10.1016/j.yjmcc.2023.03.006 |
| الإتاحة: | https://doi.org/10.1016/j.yjmcc.2023.03.006Test https://hdl.handle.net/1887/3728753Test https://www.sciencedirect.com/science/article/pii/S0022282823000512?via%3DihubTest |
| رقم الانضمام: | edsbas.98BF6724 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780022282820 0022282823 |
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| DOI: | 10.1016/j.yjmcc.2023.03.006 |