رسالة جامعية
Purificação e caracterização de proteínas de venenos de serpentes que interferem na cascata de coagulação sanguínea
| العنوان: | Purificação e caracterização de proteínas de venenos de serpentes que interferem na cascata de coagulação sanguínea |
|---|---|
| المؤلفون: | Oliveira, Daniella Gorete Lourenço de [UNESP] |
| مرشدي الرسالة: | Universidade Estadual Paulista (UNESP), Arni, Raghuvir Krishnaswamy [UNESP] |
| المصدر: | AlephRepositório Institucional da UNESPUniversidade Estadual PaulistaUNESP. |
| حالة النشر: | publishedVersion |
| بيانات النشر: | Universidade Estadual Paulista (UNESP), 2006. |
| سنة النشر: | 2006 |
| المجموعة: | IBICT Brazilian ETDs |
| Original Material: | OLIVEIRA, Daniella Gorete Lourenço de. Purificação e caracterização de proteínas de venenos de serpentes que interferem na cascata de coagulação sanguínea. 2006. 74 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas, 2006. 000489081 oliveira_dgl_me_sjrp.pdf 33004153068P9 9162508978945887 |
| مصطلحات موضوعية: | Venenos - Purificação, Sangue - Coagulação, Biologia molecular, Biofísica, Proteínas - Purificação, Enzimas proteoliticas, Proteinase, Serina proteinases, Biofísica molecular, Serpentes - Venenos, Crotalus durissus collilineatus, Crotalus durissus terrificus, Bothrops jararaca, Blood coagulation, characterization and crystallization |
| الوصف: | Made available in DSpace on 2014-06-11T19:22:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-12-08Bitstream added on 2014-06-13T20:10:06Z : No. of bitstreams: 1 oliveira_dgl_me_sjrp.pdf: 2024541 bytes, checksum: 9e617f882421a4dd2f2cc715da6fb79f (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Toxins isolated from vemos have been used as molecular tools to understand many physiological processes. The enzymes isolated from the venoms of Crotalus and Bothrops species interfere with the control and balance of the hemostatic system (PEREZ et al., 1996) and thus, the determination of their structures is potentially very important. These enzymes are serine proteinases that are similar to tyrpsin in their specificity but are generally referred to as thrombin-like enzymes due to their ability to cleave fibrinogen. The principal aim of this project was to isolate and characterize snake venom poteins that inetefere with the control and regulation of the hemostatic system in quantities and purity required for structural studies. Gel filtration, ion-exchange and HPLC chromatographic techniques were used to isolate convulxin, crotoxin, giroxin and crotamine, the principle components from the venoms of Crotalus durissus collineatus and Crotalus durissus terrificus and the serine and metalo proteinases from the venom of Bothrops jararaca. The purity of the samples was evaluated by SDS-PAGE and the specific activity of the samples was determined. Crystallization experiments were then carried out. |
| Original Identifier: | oai:repositorio.unesp.br:11449/87536 |
| نوع الوثيقة: | masterThesis |
| وصف الملف: | 74 f. : il. |
| اللغة: | Portuguese |
| العلاقة: | -1 |
| الإتاحة: | http://hdl.handle.net/11449/87536Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsndl.IBICT.oai.repositorio.unesp.br.11449.87536 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |